PMID- 28288575
OWN - NLM
STAT- MEDLINE
DCOM- 20171129
LR  - 20220408
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Mar 14
TI  - Lactobacillus-derived extracellular vesicles enhance host immune responses 
      against vancomycin-resistant enterococci.
PG  - 66
LID - 10.1186/s12866-017-0977-7 [doi]
LID - 66
AB  - BACKGROUND: Probiotic bacteria are known to modulate host immune responses 
      against various pathogens. Recently, extracellular vesicles (EVs) have emerged as 
      potentially important mediators of host-pathogen interactions. In this study, we 
      explored the role of L. plantarum derived EVs in modulating host responses to 
      vancomycin-resistant Enterococcus faecium (VRE) using both Caenorhabditis elegans 
      and human cells. RESULTS: Our previous work has shown that probiotic conditioning 
      C. elegans with L. acidophilus NCFM prolongs the survival of nematodes exposed to 
      VRE. Similarly, L. plantarum WCFS1 derived extracellular vesicles (LDEVs) also 
      significantly protected the worms against VRE infection. To dissect the molecular 
      mechanisms of this EV-induced protection, we found that treatment of C. elegans 
      with LDEVs significantly increased the transcription of host defense genes, cpr-1 
      and clec-60. Both cpr-1 and clec-60 have been previously reported to have 
      protective roles against bacterial infections. Incubating human colon-derived 
      Caco-2 cells with fluorescent dye-labeled LDEVs confirmed that LDEVs could be 
      transported into the mammalian cells. Furthermore, LDEV uptake was associated 
      with significant upregulation of CTSB, a human homologous gene of cpr-1, and 
      REG3G, a human gene that has similar functions to clec-60. CONCLUSIONS: We have 
      found that EVs produced from L. plantarum WCFS1 up-regulate the expression of 
      host defense genes and provide protective effects on hosts. Using 
      probiotic-derived EVs instead of probiotic bacteria themselves, this study 
      provides a new direction to treat antimicrobial resistant pathogens, such as VRE.
FAU - Li, Ming
AU  - Li M
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA. ming.li@brown.edu.
FAU - Lee, Kiho
AU  - Lee K
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA.
FAU - Hsu, Min
AU  - Hsu M
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA.
FAU - Nau, Gerard
AU  - Nau G
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA.
FAU - Mylonakis, Eleftherios
AU  - Mylonakis E
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA.
FAU - Ramratnam, Bharat
AU  - Ramratnam B
AD  - Division of Infectious Diseases, The Warren Alpert Medical School of Brown 
      University, Providence, RI, 02903, USA.
AD  - COBRE Center for Cancer Research, Rhode Island Hospital, Warren Alpert Medical 
      School of Brown University, Providence, RI, 02903, USA.
AD  - Clinical Research Center of Lifespan, Providence, RI, 02903, USA.
LA  - eng
GR  - K24 HD080539/HD/NICHD NIH HHS/United States
GR  - P30 GM110759/GM/NIGMS NIH HHS/United States
GR  - R01 HD072693/HD/NICHD NIH HHS/United States
GR  - U54 GM115677/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170314
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
SB  - IM
MH  - Animals
MH  - Caco-2 Cells/immunology/microbiology
MH  - Caenorhabditis elegans/genetics/immunology/microbiology
MH  - Cell Survival
MH  - Extracellular Vesicles/*immunology/*metabolism/ultrastructure
MH  - Gene Expression Regulation
MH  - Gram-Positive Bacterial Infections/microbiology
MH  - Host-Pathogen Interactions/*immunology
MH  - Humans
MH  - Lactobacillus/*metabolism
MH  - Lactobacillus plantarum/metabolism
MH  - Microscopy, Electron
MH  - Probiotics/*therapeutic use
MH  - Vancomycin-Resistant Enterococci/*immunology/*pathogenicity
PMC - PMC5348868
OTO - NOTNLM
OT  - CTSB
OT  - Extracellular vesicles
OT  - LDEVs
OT  - Lactobacillus
OT  - REG3G
OT  - VRE
OT  - Vancomycin-resistant enterococci
EDAT- 2017/03/16 06:00
MHDA- 2017/12/01 06:00
PMCR- 2017/03/14
CRDT- 2017/03/15 06:00
PHST- 2016/11/04 00:00 [received]
PHST- 2017/03/09 00:00 [accepted]
PHST- 2017/03/15 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2017/12/01 06:00 [medline]
PHST- 2017/03/14 00:00 [pmc-release]
AID - 10.1186/s12866-017-0977-7 [pii]
AID - 977 [pii]
AID - 10.1186/s12866-017-0977-7 [doi]
PST - epublish
SO  - BMC Microbiol. 2017 Mar 14;17(1):66. doi: 10.1186/s12866-017-0977-7.