PMID- 28288617 OWN - NLM STAT- MEDLINE DCOM- 20170425 LR - 20231104 IS - 1472-6882 (Electronic) IS - 1472-6882 (Linking) VI - 17 IP - 1 DP - 2017 Mar 14 TI - The effect of pomegranate fresh juice versus pomegranate seed powder on metabolic indices, lipid profile, inflammatory biomarkers, and the histopathology of pancreatic islets of Langerhans in streptozotocin-nicotinamide induced type 2 diabetic Sprague-Dawley rats. PG - 156 LID - 10.1186/s12906-017-1667-6 [doi] LID - 156 AB - BACKGROUND: Type 2 diabetes mellitus (T2DM) is associated with hyperglycemia, inflammatory disorders and abnormal lipid profiles. Several functional foods have therapeutic potential to treat chronic diseases including diabetes. The therapeutic potential of pomegranate has been stated by multitudinous scientists. The present study aimed to evaluate the effects of pomegranate juice and seed powder on the levels of plasma glucose and insulin, inflammatory biomarkers, lipid profiles, and health of the pancreatic islets of Langerhans in streptozotocin (STZ)-nicotinamide (NAD) induced T2DM Sprague Dawley (SD) rats. METHODS: Forty healthy male SD rats were induced to diabetes with a single dose intra-peritoneal administration of STZ (60 mg/kg b.w.) - NAD (120 mg/kg b.w.). Diabetic rats were orally administered with 1 mL of pomegranate fresh juice (PJ) or 100 mg pomegranate seed powder in 1 mL distilled water (PS), or 5 mg/kg b.w. of glibenclamide every day for 21 days. Rats in all groups were sacrificed on day 22. The obtained data was analyzed by SPSS software (v: 22) using One-way analysis of variance (ANOVA). RESULTS: The results showed that PJ and PS treatment had slight but non-significant reduction of plasma glucose concentration, and no impact on plasma insulin compared to diabetic control (DC) group. PJ lowered the plasma total cholesterol (TC) and triglyceride (TG) significantly, and low-density lipoproteins (LDL) non-significantly compared to DC group. In contrast, PS treatment significantly raised plasma TC, LDL, and high-density lipoproteins (HDL) levels compared to the DC rats. Moreover, the administration of PJ and PS significantly reduced the levels of plasma inflammatory biomarkers, which were actively raised in diabetic rats. Only PJ treated group showed significant repairment and restoration signs in islets of Langerhans. Besides, PJ possessed preventative impact against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals almost 2.5 folds more than PS. CONCLUSIONS: Our findings suggest that active constituents with high antioxidant properties present in PJ are responsible for its anti-hyperlipidemic and anti-inflammatory effects, likewise the restoration effect on the damaged islets of Langerhans in experimental rats. Hence, the pharmacological, biochemical, and histopathological profiles of PJ treated rats obviously indicated its helpful effects in amelioration of diabetes-associated complications. FAU - Taheri Rouhi, Seyedeh Zeinab AU - Taheri Rouhi SZ AD - Department of Nutrition & Dietetics, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia. taheri.ghazal87@gmail.com. FAU - Sarker, Md Moklesur Rahman AU - Sarker MMR AD - Department of Pharmacology, Faculty of Pharmacy, Lincoln University College, No. 2, Jalan Stadium SS 7/15, Kalana Jaya, 47301, Petaling Jaya, Selangor, Malaysia. moklesur2002@yahoo.com. AD - Department of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka, 1205, Bangladesh. moklesur2002@yahoo.com. FAU - Rahmat, Asmah AU - Rahmat A AD - Department of Nutrition & Dietetics, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia. FAU - Alkahtani, Saad Ahmed AU - Alkahtani SA AD - Department of Pharmacolgy, College of Pharmacy, Najran University, Najran, Kingdom of Saudi Arabia. FAU - Othman, Fauziah AU - Othman F AD - Department of Human Anatomy, Faculty of Medicine & Health Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia. LA - eng PT - Journal Article DEP - 20170314 PL - England TA - BMC Complement Altern Med JT - BMC complementary and alternative medicine JID - 101088661 RN - 0 (Biomarkers) RN - 0 (Hypoglycemic Agents) RN - 0 (Insulin) RN - 0 (Lipoproteins) RN - 0 (Plant Extracts) RN - 0 (Triglycerides) RN - 25X51I8RD4 (Niacinamide) RN - 5W494URQ81 (Streptozocin) RN - 97C5T2UQ7J (Cholesterol) SB - IM EIN - BMC Complement Altern Med. 2017 Apr 13;17 (1):214. PMID: 28407770 MH - Animals MH - Biomarkers/blood MH - Cholesterol/blood MH - Diabetes Mellitus, Type 2/blood/*diet therapy/*drug therapy/pathology MH - Disease Models, Animal MH - Fruit and Vegetable Juices/*analysis MH - Humans MH - Hypoglycemic Agents/*administration & dosage MH - Insulin/blood MH - Islets of Langerhans/drug effects/metabolism/pathology MH - Lipoproteins/blood MH - Lythraceae/*chemistry MH - Male MH - Niacinamide MH - Plant Extracts/*administration & dosage MH - Rats MH - Rats, Sprague-Dawley MH - Seeds/chemistry MH - Streptozocin MH - Triglycerides/blood PMC - PMC5348881 OTO - NOTNLM OT - Anti-hyperlipidemic OT - Anti-inflammatory OT - Anti-oxidant OT - Diabetes mellitus OT - Pancreatic health OT - Plasma glucose OT - Pomegranate juice OT - Pomegranate seed powder EDAT- 2017/03/16 06:00 MHDA- 2017/04/26 06:00 PMCR- 2017/03/14 CRDT- 2017/03/15 06:00 PHST- 2016/11/09 00:00 [received] PHST- 2017/03/07 00:00 [accepted] PHST- 2017/03/15 06:00 [entrez] PHST- 2017/03/16 06:00 [pubmed] PHST- 2017/04/26 06:00 [medline] PHST- 2017/03/14 00:00 [pmc-release] AID - 10.1186/s12906-017-1667-6 [pii] AID - 1667 [pii] AID - 10.1186/s12906-017-1667-6 [doi] PST - epublish SO - BMC Complement Altern Med. 2017 Mar 14;17(1):156. doi: 10.1186/s12906-017-1667-6.