PMID- 28288912 OWN - NLM STAT- MEDLINE DCOM- 20170628 LR - 20170628 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 64 DP - 2017 May TI - Anti-inflammatory and hepatoprotective effects of glycyrrhetinic acid on CCl(4)-induced damage in precision-cut liver slices from Jian carp (Cyprinus carpio var. jian) through inhibition of the nf-kB pathway. PG - 234-242 LID - S1050-4648(17)30126-2 [pii] LID - 10.1016/j.fsi.2017.03.007 [doi] AB - In order to evaluate the antioxidant and anti-inflammatory effects of glycyrrhetinic acid (GA) on carbon tetrachloride (CCl(4))-induced damage in precision-cut liver slices (PCLS) from Jian carp (Cyprinus carpio. Jian), an acute liver damage model was established in this study. The viability of PCLS, levels of anti-oxidases in liver homogenates, expression of inflammation-related genes including nuclear factor-kappaB (nf-kappaB)/c-rel, inducible nitric oxide synthase (inos), interleukin-1beta (il-1beta), interleukin-6 (il-6) and interleukin-8 (il-8), and protein levels of (nf-kappaB)/c-rel in liver tissues were measured. The results showed that pretreatment of PCLS with GA at 5 and 10 mug/mL for 6 h significantly inhibited the cytotoxicity of CCl(4). GA attenuated CCl(4)-induced oxidative stress in PCLS through promoting the recovery of superoxide dismutase (SOD) and glutathione (GSH) levels, and inhibiting malondialdehyde (MDA) synthesis. In inflammatory response, GA at both 5 and 10 mug/mL significantly inhibited the increase in mRNA levels of inflammatory cytokines including nf-kB/c-rel, inos, il-1beta, il-6 and il-8, and the protein level of Nf-kB/C-rel induced by CCl(4). Furthermore, treatment with pyrrolyl dithiocarbamate (PDTC, 4 mug/mL), an inhibitor of nuclear transcription factor nf-kB, significantly inhibited nf-kB levels, and transcription of downstream cytokines inos, il-1beta, il-6 and il-8, also the viability of PCLS was significantly increased. These results indicated that GA suppressed inflammation and reduced cytotoxicity by inhibiting the nf-kB signaling pathway, and plays a role in liver protection. CI - Copyright (c) 2017. Published by Elsevier Ltd. FAU - Cao, Liping AU - Cao L AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Ding, Weidong AU - Ding W AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Jia, Rui AU - Jia R AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Du, Jingliang AU - Du J AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Wang, Tao AU - Wang T AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Zhang, Chunyun AU - Zhang C AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Gu, Zhengyan AU - Gu Z AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Yin, Guojun AU - Yin G AD - International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: yingj@ffrc.cn. LA - eng PT - Journal Article DEP - 20170310 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Antioxidants) RN - 0 (Fish Proteins) RN - 0 (NF-kappa B) RN - CL2T97X0V0 (Carbon Tetrachloride) RN - P540XA09DR (Glycyrrhetinic Acid) SB - IM MH - Animals MH - Anti-Inflammatory Agents/*pharmacology MH - Antioxidants/*pharmacology MH - Carbon Tetrachloride/toxicity MH - Carps/*genetics/*immunology MH - Dose-Response Relationship, Drug MH - Fish Proteins/metabolism MH - Glycyrrhetinic Acid/*pharmacology MH - Liver/*enzymology MH - NF-kappa B/metabolism MH - Signal Transduction/drug effects OTO - NOTNLM OT - CCl(4) OT - Cyprinus carpio var. jian OT - Glycyrrhetinic acid OT - Inflammatory cytokines OT - Nuclear factor-kappaB OT - Precision-cut liver slices EDAT- 2017/03/16 06:00 MHDA- 2017/06/29 06:00 CRDT- 2017/03/15 06:00 PHST- 2016/10/12 00:00 [received] PHST- 2017/03/02 00:00 [revised] PHST- 2017/03/03 00:00 [accepted] PHST- 2017/03/16 06:00 [pubmed] PHST- 2017/06/29 06:00 [medline] PHST- 2017/03/15 06:00 [entrez] AID - S1050-4648(17)30126-2 [pii] AID - 10.1016/j.fsi.2017.03.007 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2017 May;64:234-242. doi: 10.1016/j.fsi.2017.03.007. Epub 2017 Mar 10.