PMID- 28289517
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1949-8454 (Print)
IS  - 1949-8454 (Electronic)
IS  - 1949-8454 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Feb 26
TI  - Retroviral integrase protein and intasome nucleoprotein complex structures.
PG  - 32-44
LID - 10.4331/wjbc.v8.i1.32 [doi]
AB  - Retroviral replication proceeds through the integration of a DNA copy of the 
      viral RNA genome into the host cellular genome, a process that is mediated by the 
      viral integrase (IN) protein. IN catalyzes two distinct chemical reactions: 
      3'-processing, whereby the viral DNA is recessed by a di- or trinucleotide at its 
      3'-ends, and strand transfer, in which the processed viral DNA ends are inserted 
      into host chromosomal DNA. Although IN has been studied as a recombinant protein 
      since the 1980s, detailed structural understanding of its catalytic functions 
      awaited high resolution structures of functional IN-DNA complexes or intasomes, 
      initially obtained in 2010 for the spumavirus prototype foamy virus (PFV). Since 
      then, two additional retroviral intasome structures, from the alpha-retrovirus Rous 
      sarcoma virus (RSV) and beta-retrovirus mouse mammary tumor virus (MMTV), have 
      emerged. Here, we briefly review the history of IN structural biology prior to 
      the intasome era, and then compare the intasome structures of PFV, MMTV and RSV 
      in detail. Whereas the PFV intasome is characterized by a tetrameric assembly of 
      IN around the viral DNA ends, the newer structures harbor octameric IN 
      assemblies. Although the higher order architectures of MMTV and RSV intasomes 
      differ from that of the PFV intasome, they possess remarkably similar intasomal 
      core structures. Thus, retroviral integration machineries have adapted 
      evolutionarily to utilize disparate IN elements to construct convergent intasome 
      core structures for catalytic function.
FAU - Grawenhoff, Julia
AU  - Grawenhoff J
AD  - Julia Grawenhoff, Alan N Engelman, Department of Cancer Immunology and Virology, 
      Dana-Farber Cancer Institute, Boston, MA 02215, United States.
FAU - Engelman, Alan N
AU  - Engelman AN
AD  - Julia Grawenhoff, Alan N Engelman, Department of Cancer Immunology and Virology, 
      Dana-Farber Cancer Institute, Boston, MA 02215, United States.
LA  - eng
GR  - R01 AI070042/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Review
PL  - United States
TA  - World J Biol Chem
JT  - World journal of biological chemistry
JID - 101546471
PMC - PMC5329712
OTO - NOTNLM
OT  - 3-dimensional structure
OT  - DNA integration
OT  - Human immunodeficiency virus/acquired immune deficiency syndrome
OT  - Intasome
OT  - Integrase
OT  - Mouse mammary tumor virus
OT  - Prototype foamy virus
OT  - Retrovirus
OT  - Rous sarcoma virus
COIS- Conflict-of-interest statement: Authors declare no conflicts of interest for this 
      article.
EDAT- 2017/03/16 06:00
MHDA- 2017/03/16 06:01
PMCR- 2017/02/26
CRDT- 2017/03/15 06:00
PHST- 2016/11/01 00:00 [received]
PHST- 2016/12/24 00:00 [revised]
PHST- 2017/01/11 00:00 [accepted]
PHST- 2017/03/15 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2017/03/16 06:01 [medline]
PHST- 2017/02/26 00:00 [pmc-release]
AID - 10.4331/wjbc.v8.i1.32 [doi]
PST - ppublish
SO  - World J Biol Chem. 2017 Feb 26;8(1):32-44. doi: 10.4331/wjbc.v8.i1.32.