PMID- 28290371
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20190304
IS  - 1873-7145 (Electronic)
IS  - 0963-9969 (Linking)
VI  - 94
DP  - 2017 Apr
TI  - Release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from milk protein 
      isolate (MPI) during enzymatic hydrolysis.
PG  - 79-89
LID - S0963-9969(17)30056-X [pii]
LID - 10.1016/j.foodres.2017.02.004 [doi]
AB  - The release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from bovine 
      milk protein isolate (MPI) during trypsin hydrolysis was studied using a design 
      of experiments (DOE) approach. A 3 factorx3 level DOE including temperature (40, 
      50 and 60 degrees C), enzyme to substrate ratio (E:S; 0.50, 1.25 and 2.00% (w/w)) and 
      hydrolysis time (60, 150 and 240min) was used during the generation of 15 
      hydrolysates (H1-H15). The degree of hydrolysis (DH) varied between 6.98+/-0.31 
      (H8) to 12.75+/-0.62% (H10). The DPP-IV half maximal inhibitory concentration 
      (IC(50)) ranged from 0.68+/-0.06 (H11)/0.68+/-0.10 (H4) to 1.59+/-0.11mgmL(-1) (H8). 
      Temperature had no effect (p>0.05) on the DPP-IV IC(50) value, while an increase 
      in E:S or time significantly decreased DPP-IV IC(50) value (p<0.05). The DPP-IV 
      IC(50) value of 0.69mgmL(-1), predicted by response surface methodology (RSM), to 
      be obtained with an hydrolysate generated at 50.5 degrees C, 2% ES and 231min (H16) was 
      similar to the experimentally obtained value (DPP-IV IC(50)=0.66+/-0.10mgmL(-1), 
      p>0.05, n=3). Following simulated gastrointestinal digestion (SGID) of H16 
      (H16_CorPP), the DPP-IV IC(50) value increased (p<0.05) to 0.90+/-0.07mgmL(-1). 
      There was no significant difference between the DPP-IV IC(50) value of the SGID 
      of MPI (MPI_CorPP, 0.89+/-0.11mgmL(-1)) and that of H16_CorPP. Potent known DPP-IV 
      inhibitory peptide sequences were identified by liquid chromatography-tandem mass 
      spectrometry (LC-MS/MS) within H16, some of which were also present within 
      H16_CorPP. MPI hydrolysates may be of interest for serum glucose regulation in 
      humans.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Nongonierma, Alice B
AU  - Nongonierma AB
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland.
FAU - Mazzocchi, Caterina
AU  - Mazzocchi C
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland.
FAU - Paolella, Sara
AU  - Paolella S
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland.
FAU - FitzGerald, Richard J
AU  - FitzGerald RJ
AD  - Department of Biological Sciences, University of Limerick, Limerick, Ireland; 
      Food for Health Ireland (FHI), University of Limerick, Limerick, Ireland. 
      Electronic address: dick.fitzgerald@ul.ie.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170209
PL  - Canada
TA  - Food Res Int
JT  - Food research international (Ottawa, Ont.)
JID - 9210143
RN  - 0 (Dipeptidyl-Peptidase IV Inhibitors)
RN  - 0 (Milk Proteins)
RN  - 0 (Peptides)
RN  - 0 (Protein Hydrolysates)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - *Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Digestion
MH  - Dipeptidyl Peptidase 4/*metabolism
MH  - Dipeptidyl-Peptidase IV Inhibitors/*analysis
MH  - Humans
MH  - Hydrolysis
MH  - Milk Proteins/*metabolism
MH  - Peptides/*analysis
MH  - Protein Hydrolysates/*metabolism
MH  - Tandem Mass Spectrometry
MH  - Trypsin/*metabolism
OTO - NOTNLM
OT  - Bioactive peptides
OT  - Dipeptidyl peptidase IV inhibition
OT  - Milk protein isolate
OT  - Response surface methodology
OT  - Trypsin
EDAT- 2017/03/16 06:00
MHDA- 2019/03/05 06:00
CRDT- 2017/03/15 06:00
PHST- 2016/12/08 00:00 [received]
PHST- 2017/01/30 00:00 [revised]
PHST- 2017/02/05 00:00 [accepted]
PHST- 2017/03/15 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
AID - S0963-9969(17)30056-X [pii]
AID - 10.1016/j.foodres.2017.02.004 [doi]
PST - ppublish
SO  - Food Res Int. 2017 Apr;94:79-89. doi: 10.1016/j.foodres.2017.02.004. Epub 2017 
      Feb 9.