PMID- 28292010
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20180226
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 89
DP  - 2017 May
TI  - The anti-allergic and anti-inflammatory effects of Benjakul extract (a Thai 
      traditional medicine), its constituent plants and its some pure constituents 
      using in vitro experiments.
PG  - 1018-1026
LID - S0753-3322(16)31797-8 [pii]
LID - 10.1016/j.biopha.2017.02.066 [doi]
AB  - Benjakul (BJK), a Thai traditional medicine preparation, has long been used for 
      balanced health, controlled abnormal of element in the body, carminative, and 
      relief of flatulence. It is composed of five plants: Piper interruptum Opiz., 
      Piper longum L., Piper sarmentosum Roxb., Plumbago indica L., and Zingiber 
      officinale Roscoe. The ethanolic extracts of BJK, its five individual plants, and 
      pure constituents of BJK were investigated for their anti-allergic activity using 
      immunoglobulin E (IgE)-sensitized beta-hexosaminidase in the rat basophilic 
      leukemia-2H3 (RBL-2H3) cells and anti-inflammatory activity using 
      lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis 
      factor-alpha (TNF-alpha) in the murine macrophage (RAW 264.7) cells. The ethanolic 
      extracts of BJK showed anti-allergic activity (IC(50)=12.69mug/ml) and exhibited 
      potent NO inhibitory effect (IC(50)=16.60mug/ml), but inactive on TNF-alpha release. 
      Moreover, 6-shogaol and plumbagin, two pure compounds from BJK, showed higher 
      anti-allergic activity than the ethanolic BJK extract with IC(50) values of 0.28 
      and 4.03mug/ml, respectively. These compounds were significantly higher than 
      chlorpheniramine (CPM), standard drug, with IC(50) value of 17.98mug/ml. 
      Determination of the anti-inflammatory activity by measuring the inhibition of NO 
      production presented that plumbagin and 6-shogaol exhibited higher than crude BJK 
      extract with IC(50) values of 0.002 and 0.92mug/ml, respectively. In particular, 
      plumbagin also showed higher anti-inflammatory than prednisolone, positive 
      control, with IC(50) value of 0.59mug/ml. 6-Shogaol also showed inhibitory effect 
      on TNF-alpha release (IC(50)=9.16mug/ml). These preliminary results may provide some 
      scientific support for the use of BJK for the anti-allergic treatment and 
      inflammatory disorders through the inhibition of NO production.
CI  - Copyright (c) 2017 Elsevier Masson SAS. All rights reserved.
FAU - Makchuchit, Sunita
AU  - Makchuchit S
AD  - Graduate School, Faculty of Medicine, Thammasat University, Klongluang, 
      Pathumthani 12120, Thailand. Electronic address: msunita_359@hotmail.com.
FAU - Rattarom, Ruchilak
AU  - Rattarom R
AD  - Faculty of Pharmacy, Mahasarakham University, Kantarawichai, Maha Sarakham 44150, 
      Thailand. Electronic address: rujiluk_99@yahoo.com.
FAU - Itharat, Arunporn
AU  - Itharat A
AD  - Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat 
      University, Klongluang, Pathumthani 12120, Thailand; Center of Excellence in 
      Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, 
      Klongluang, Pathumthani 12120, Thailand. Electronic address: iarunporn@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20170310
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Lipopolysaccharides/toxicity
MH  - Medicine, Traditional
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plants, Medicinal/*chemistry
MH  - Rats
MH  - Thailand
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - beta-N-Acetylhexosaminidases/genetics/metabolism
OTO - NOTNLM
OT  - Anti-allergy
OT  - Anti-inflammation
OT  - Benjakul
OT  - RAW264.7
OT  - Thai traditional medicine
OT  - beta-Hexosaminidase
EDAT- 2017/03/16 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/03/16 06:00
PHST- 2016/09/30 00:00 [received]
PHST- 2017/01/31 00:00 [revised]
PHST- 2017/02/14 00:00 [accepted]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
PHST- 2017/03/16 06:00 [entrez]
AID - S0753-3322(16)31797-8 [pii]
AID - 10.1016/j.biopha.2017.02.066 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2017 May;89:1018-1026. doi: 10.1016/j.biopha.2017.02.066. 
      Epub 2017 Mar 10.