PMID- 28293296
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 10
DP  - 2017
TI  - Isolation and characterization of chromosomal markers in Poa pratensis.
PG  - 5
LID - 10.1186/s13039-017-0307-7 [doi]
LID - 5
AB  - BACKGROUND: Poa pratensis L. is a turf grass and forage crop used worldwide. 
      Being a facultative apomictic species, P. pratensis has a highly variable 
      chromosome number. Chromosomal markers constitute a powerful tool for chromosome 
      identification and for various aspects of genomic research. However, currently, 
      no chromosomal markers are available for P. pratensis. RESULTS: Four novel 
      chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined 
      with fluorescence in situ hybridization (FISH) in Poa pratensis. Three tandemly 
      repetitive sequences (PpTR-1, PpTR-2, and PpTR-3) were characterized as 
      subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in PpTR-1, 
      PpTR-2, and PpTR-3, respectively. One tandemly repetitive sequence (PpCR-1) was 
      shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The 
      distribution patterns of PpTR-1, PpTR-2, and PpTR-3 were highly conserved across 
      different P. pratensis cultivars and in the distantly related Poa species, 
      whereas PpCR-1 was conserved across different P. pratensis cultivars, but less 
      conserved across Poa species. CONCLUSION: In this study, we report the 
      identification and characterization of four novel chromosomal markers in P. 
      pratensis. These chromosomal markers are powerful tools for accurate assessment 
      of chromosome count, genomic and phylogenetic analyses, as well as studies of 
      apomixis in P. pratensis.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, the Chinese Academy of Sciences, Xining, 810008 China. ISNI: 
      0000 0004 1769 9989. GRID: grid.458496.2
FAU - Yu, Feng
AU  - Yu F
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, the Chinese Academy of Sciences, Xining, 810008 China. ISNI: 
      0000 0004 1769 9989. GRID: grid.458496.2
FAU - Liu, Ruijuan
AU  - Liu R
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, the Chinese Academy of Sciences, Xining, 810008 China. ISNI: 
      0000 0004 1769 9989. GRID: grid.458496.2
FAU - Dou, Quanwen
AU  - Dou Q
AUID- ORCID: 0000-0001-5910-6270
AD  - Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute 
      of Plateau Biology, the Chinese Academy of Sciences, Xining, 810008 China. ISNI: 
      0000 0004 1769 9989. GRID: grid.458496.2
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC5345224
OTO - NOTNLM
OT  - Chromosomal markers
OT  - Poa pratensis
OT  - Repetitive sequence
EDAT- 2017/03/16 06:00
MHDA- 2017/03/16 06:01
PMCR- 2017/03/09
CRDT- 2017/03/16 06:00
PHST- 2016/11/02 00:00 [received]
PHST- 2017/02/21 00:00 [accepted]
PHST- 2017/03/16 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2017/03/16 06:01 [medline]
PHST- 2017/03/09 00:00 [pmc-release]
AID - 307 [pii]
AID - 10.1186/s13039-017-0307-7 [doi]
PST - epublish
SO  - Mol Cytogenet. 2017 Mar 9;10:5. doi: 10.1186/s13039-017-0307-7. eCollection 2017.