PMID- 28294371
OWN - NLM
STAT- MEDLINE
DCOM- 20180201
LR  - 20220311
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 50
IP  - 1
DP  - 2018 Feb
TI  - Icariin improves the sexual function of male mice through the PI3K/AKT/eNOS/NO 
      signalling pathway.
LID - 10.1111/and.12802 [doi]
AB  - We aimed to investigate the effect and mechanism of icariin on male sexual 
      function. Forty-eight Crl:CD1(ICR) male mice were randomly divided into control, 
      low-, medium- and high-dose icariin group (intragastric administration of 50, 100 
      and 200 mg/kg/d for 21 days). Mating experiment was then performed at a ratio of 
      1: 3 (male: female). The mating behaviours of male mice were recorded. The 
      genital indexes and serum testosterone, nitric oxide (NO), hypothalamic dopamine 
      (DA) and 5- hydroxy tryptophan (5-HT) concentrations were measured. The 
      expression of endothelial nitric oxide (eNOS), phosphatidylinositol tallow 
      alcohol 3-kinase (PI3K) and phosphorylated protein kinase (p-AKT) in penile 
      tissue was detected by Western blot. All icariin groups exhibited shorter capture 
      latency and ejaculation latency, increased number of capture and ejaculation, 
      higher capture and ejaculation rate, and higher testicular and prostate indexes 
      compared with controls (p < .001). These groups had higher serum testosterone and 
      NO concentrations (p < .001), hypothalamic DA and 5-HT levels, and eNOS, PI3K and 
      phosphorylated AKT expressions in penile tissue (p < .05). The effect of icariin 
      was dose-dependently increased. Our study suggests that icariin improves the 
      sexual function of male mice, which might be associated with the 
      hypothalamic-pituitary-gonadal axis and the PI3K/AKT/eNOS/NO signalling pathway.
CI  - (c) 2017 Blackwell Verlag GmbH.
FAU - Ding, J
AU  - Ding J
AD  - Department of Urology, The Third Xiang Ya Hospital/Central South University, 
      Changsha City, Hunan Province, 410013, China.
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
FAU - Tang, Y
AU  - Tang Y
AD  - Department of Urology, The Third Xiang Ya Hospital/Central South University, 
      Changsha City, Hunan Province, 410013, China.
FAU - Tang, Z
AU  - Tang Z
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
FAU - Zu, X
AU  - Zu X
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
FAU - Qi, L
AU  - Qi L
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
FAU - Wang, G
AU  - Wang G
AD  - Department of Urology, Xiang Ya Hospital/Central South University, Changsha City, 
      Hunan Province, 410008, China.
LA  - eng
PT  - Journal Article
DEP - 20170315
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Ejaculation/*drug effects
MH  - Flavonoids/*pharmacology
MH  - Male
MH  - Mice
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Penis/*drug effects/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Sexual Behavior, Animal/*drug effects
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - Icariin
OT  - PI3K/AKT/eNOS/NO signalling pathway
OT  - nitric oxide
OT  - sexual function
OT  - testosterone
EDAT- 2017/03/16 06:00
MHDA- 2018/02/02 06:00
CRDT- 2017/03/16 06:00
PHST- 2016/12/21 00:00 [accepted]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2018/02/02 06:00 [medline]
PHST- 2017/03/16 06:00 [entrez]
AID - 10.1111/and.12802 [doi]
PST - ppublish
SO  - Andrologia. 2018 Feb;50(1). doi: 10.1111/and.12802. Epub 2017 Mar 15.