PMID- 28296571
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20200930
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 16
IP  - 9
DP  - 2017 May 3
TI  - Efficient generation of functional Schwann cells from adipose-derived stem cells 
      in defined conditions.
PG  - 841-851
LID - 10.1080/15384101.2017.1304328 [doi]
AB  - Schwann cells (SCs) are hitherto regarded as the most promising candidates for 
      viable cell-based therapy to peripheral nervous system (PNS) injuries or 
      degenerative diseases. However, the extreme drawbacks of transplanting autologous 
      SCs for clinical applications still represent a significant bottleneck in neural 
      regenerative medicine, mainly owing to the need of sacrificing a functional nerve 
      to generate autologous SCs and the nature of slow expansion of the SCs. Thus, it 
      is of great importance to establish an alternative cell system for the generation 
      of sufficient SCs. Here, we demonstrated that adipose-derived stem cells (ADSCs) 
      of rat robustly give rise to morphological, phenotypic and functional SCs using 
      an optimized protocol. After undergoing a 3-week in vitro differentiation, almost 
      all of treated ADSCs exhibited spindle shaped morphology similar to genuine SCs 
      and expressed SC markers GFAP and S100. Most importantly, apart from acquisition 
      of SC antigenic and biochemical features, the ADSC-derived SCs were functionally 
      identical to native SCs as they possess a potential ability to form myelin, and 
      secret nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and 
      glia-derived neurotrophic factor (GDNF). The current study may provide an ideal 
      strategy for harvesting sufficient SCs for cell-based treatment of various 
      peripheral nerve injuries or disorders.
FAU - Xie, Songtao
AU  - Xie S
AD  - a Department of Burn Surgery , Xijing Hospital, Fourth Military Medical 
      University, Xi'an PR China State Key Laboratory of Cancer Biology, Xijing 
      Hospital Fourth Military Medical University , Xi'an , Shaanxi Province , China.
FAU - Lu, Fan
AU  - Lu F
AD  - b Department of Biochemistry and Molecular Biology , China State Key Laboratory 
      of Cancer Biology, Fourth Military Medical University , Xi'an , Shaanxi Province 
      , China.
FAU - Han, Juntao
AU  - Han J
AD  - a Department of Burn Surgery , Xijing Hospital, Fourth Military Medical 
      University, Xi'an PR China State Key Laboratory of Cancer Biology, Xijing 
      Hospital Fourth Military Medical University , Xi'an , Shaanxi Province , China.
FAU - Tao, Ke
AU  - Tao K
AD  - a Department of Burn Surgery , Xijing Hospital, Fourth Military Medical 
      University, Xi'an PR China State Key Laboratory of Cancer Biology, Xijing 
      Hospital Fourth Military Medical University , Xi'an , Shaanxi Province , China.
FAU - Wang, Hongtao
AU  - Wang H
AD  - a Department of Burn Surgery , Xijing Hospital, Fourth Military Medical 
      University, Xi'an PR China State Key Laboratory of Cancer Biology, Xijing 
      Hospital Fourth Military Medical University , Xi'an , Shaanxi Province , China.
FAU - Simental, Alfred
AU  - Simental A
AD  - c Department of Otolaryngology-Head and Neck Surgery , Loma Linda University 
      Medical Center , Loma Linda , CA , USA.
FAU - Hu, Dahai
AU  - Hu D
AD  - a Department of Burn Surgery , Xijing Hospital, Fourth Military Medical 
      University, Xi'an PR China State Key Laboratory of Cancer Biology, Xijing 
      Hospital Fourth Military Medical University , Xi'an , Shaanxi Province , China.
FAU - Yang, Hao
AU  - Yang H
AD  - d Translational Medicine Center , Hong Hui Hospital, Xi'an Jiaotong University , 
      Xi'an , Shaanxi , China.
LA  - eng
PT  - Journal Article
DEP - 20170315
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Myelin Sheath/metabolism
MH  - Nerve Growth Factors/metabolism
MH  - Olfactory Bulb/cytology
MH  - Phenotype
MH  - Rats
MH  - Schwann Cells/*cytology/metabolism
MH  - Stem Cells/*cytology/metabolism
PMC - PMC5444349
OTO - NOTNLM
OT  - Schwann cells
OT  - adipose-derived stem cells
OT  - cell differentiation
OT  - myelination
OT  - peripheral nerve
EDAT- 2017/03/16 06:00
MHDA- 2018/02/24 06:00
PMCR- 2018/03/15
CRDT- 2017/03/16 06:00
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2017/03/16 06:00 [entrez]
PHST- 2018/03/15 00:00 [pmc-release]
AID - 1304328 [pii]
AID - 10.1080/15384101.2017.1304328 [doi]
PST - ppublish
SO  - Cell Cycle. 2017 May 3;16(9):841-851. doi: 10.1080/15384101.2017.1304328. Epub 
      2017 Mar 15.