PMID- 28297580
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20190705
IS  - 2157-6564 (Print)
IS  - 2157-6580 (Electronic)
IS  - 2157-6564 (Linking)
VI  - 6
IP  - 3
DP  - 2017 Mar
TI  - A Human Corneal Epithelial Cell Line Model for Limbal Stem Cell Biology and 
      Limbal Immunobiology.
PG  - 761-766
LID - 10.5966/sctm.2016-0175 [doi]
AB  - Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by 
      abnormal maintenance of LSCs. It is treated by transplantation of donor-derived 
      limbal epithelial cells (LECs), the success of which depends on the presence and 
      quality of LSCs within the transplant. Understanding the immunobiological 
      responses of these cells within the transplants could improve cell engraftment 
      and survival. However, human corneal rings used as a source of LSCs are not 
      always readily available for research purposes. As an alternative, we 
      hypothesized that a human telomerase-immortalized corneal epithelial cell (HTCEC) 
      line could be used as a model for studying LSC immunobiology. HTCEC 
      constitutively expressed human leukocyte antigen (HLA) class I but not class II 
      molecules. However, when stimulated by interferon-gamma, HTCECs then expressed HLA 
      class II antigens. Some HTCECs were also migratory in response to CXCL12 and 
      expressed stem cell markers, Nanog, Oct4, and Sox2. In addition because both 
      HTCECs and LECs contain side population (SP) cells, which are an enriched LSC 
      population, we used these SP cells to show that some HTCEC SP cells coexpressed 
      ABCG2 and ABCB5. HTCEC SP and non-side population (NSP) cells also expressed 
      CXCR4, but the SP cells expressed higher levels. Both were capable of colony 
      formation, but the NSP colonies were smaller and contained fewer cells. In 
      addition, HTCECs expressed DeltaNp63alpha. These results suggest the HTCEC line is a 
      useful model for further understanding LSC biology by using an in vitro approach 
      without reliance on a supply of human tissue. Stem Cells Translational Medicine 
      2017;6:761-766.
CI  - (c) 2016 The Authors Stem Cells Translational Medicine published by Wiley 
      Periodicals, Inc. on behalf of AlphaMed Press.
FAU - Shaharuddin, Bakiah
AU  - Shaharuddin B
AD  - Institute of Genetic Medicine, Newcastle University, Newcastle Upon-Tyne, United 
      Kingdom.
AD  - Advanced Medical and Dental Institute, Universiti Sains Malaysia, Pulau Pinang, 
      Malaysia.
FAU - Ahmad, Sajjad
AU  - Ahmad S
AD  - St. Paul's Eye Unit, Royal Liverpool University Hospital, Liverpool, United 
      Kingdom.
AD  - Department of Eye and Vision Sciences, Institute of Ageing and Chronic Disease, 
      University of Liverpool, Liverpool, United Kingdom.
FAU - Md Latar, Nani
AU  - Md Latar N
AD  - Institute of Genetic Medicine, Newcastle University, Newcastle Upon-Tyne, United 
      Kingdom.
AD  - Department of Surgery, Faculty of Medicine, Universiti Kebangsaan, Malaysia 
      Medical Centre, Kuala Lumpur, Malaysia.
FAU - Ali, Simi
AU  - Ali S
AD  - Institute of Cellular Medicine, Newcastle University, Newcastle Upon-Tyne, United 
      Kingdom.
FAU - Meeson, Annette
AU  - Meeson A
AD  - Institute of Genetic Medicine, Newcastle University, Newcastle Upon-Tyne, United 
      Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161014
PL  - England
TA  - Stem Cells Transl Med
JT  - Stem cells translational medicine
JID - 101578022
RN  - 0 (Biomarkers)
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, CXCR4)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - Chemotaxis
MH  - Colony-Forming Units Assay
MH  - Epithelial Cells/cytology/metabolism
MH  - Epithelium, Corneal/*cytology
MH  - HLA Antigens/metabolism
MH  - Histocompatibility Testing
MH  - Humans
MH  - Limbus Corneae/*cytology/*immunology
MH  - Mice
MH  - *Models, Biological
MH  - Receptors, CXCR4/metabolism
MH  - Side-Population Cells/cytology
MH  - Stem Cells/*cytology/metabolism
MH  - Telomerase/metabolism
PMC - PMC5442771
OTO - NOTNLM
OT  - ABCB5
OT  - CXCR4
OT  - Immunobiology
OT  - Limbal stem cell
OT  - Side population
EDAT- 2017/03/16 06:00
MHDA- 2019/07/06 06:00
PMCR- 2017/03/01
CRDT- 2017/03/16 06:00
PHST- 2016/04/06 00:00 [received]
PHST- 2016/09/01 00:00 [accepted]
PHST- 2017/03/16 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - SCT312114 [pii]
AID - 10.5966/sctm.2016-0175 [doi]
PST - ppublish
SO  - Stem Cells Transl Med. 2017 Mar;6(3):761-766. doi: 10.5966/sctm.2016-0175. Epub 
      2016 Oct 14.