PMID- 28297833
OWN - NLM
STAT- MEDLINE
DCOM- 20180914
LR  - 20231213
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 97
IP  - 9
DP  - 2017 Mar 7
TI  - [Effect of allogenic mesenchymal stem cells transplantation on the expression of 
      interleukin-22 and matrix metalloproteinase-3 in rats with collagen induced 
      arthritis].
PG  - 698-702
LID - 10.3760/cma.j.issn.0376-2491.2017.09.014 [doi]
AB  - Objective: To study the effect of allogeneic bone marrow mesenchymal stem cells 
      transplantation on the expression of interleukin -22 (IL-22), matrix 
      metalloproteinase -3 (MMP-3) in serum and synovial of rats with collagen induced 
      arthritis. Methods: Type Ⅱ collagen were injected twice to establish the collagen 
      induced arthritis (CIA) rat model. Forty-eight rats were randomly divided into 3 
      groups: control group, CIA control group, CIA experiment group. Rat bone marrow 
      mesenchymal stem cells were cultured by bone marrow method combined with adherent 
      culture method. After identify, the remaining cells were injected in the CIA 
      experimental group. Enzyme linked immunosorbent assay (ELISA) and 
      immunohistochemistry were used to detect the expression of IL-22 and MMP-3 in 
      serum and anklebone joint's synovium of rats, respectively. Synovial cells were 
      isolated and cultured, and were treated with different concentrations of IL-22. 
      MMP-3 protein and mRNA were detected before and after stimulation by Western blot 
      and real-time quantitative polymerase chain reaction (RT-qPCR). Results: After 
      MSC transplantation, arthritis index, X-ray, HE staining of CIA rat showed that 
      joint damage significantly reduced compared with the control group. The ELISA 
      results showed that the expression of MMP-3 and IL-22 in CIA control group was 
      higher than those in the control group (125.79+/-9.12 vs 102.00+/-7.63 ng/ml, 
      P<0.05), (292.35+/-31.23 vs 257.27+/-13.99 ng/ml, P<0.05) and CIA experiment group 
      (125.79+/-9.12 vs 97.94+/-9.50 ng/ml, P<0.05), (292.35+/-31.23 vs 262.16+/-22.02 ng/ml, 
      P<0.05) with statistically significant difference (P<0.05). There was no 
      significant difference between the control group and CIA experimental group. 
      Immunohistochemical showed similar results with ELISA. Western blotting and 
      RT-qPCR showed that MMP-3 protein and mRNA expression was increased after IL-22 
      stimulation in a dose-dependent manner. Conclusion: IL-22 and MMP-3 play an 
      important role in the pathogenesis of rheumatoid arthritis. IL-22 could regulate 
      the expression of MMP-3, and bone marrow mesenchymal stem cells could reduce the 
      expression of MMP-3 in the treatment of rheumatoid arthritis by reducing the 
      expression of IL-22.
FAU - Liu, G Y
AU  - Liu GY
AD  - Department of Rheumatism, the Second Hospital of Shanxi Medical University, 
      Taiyuan 030001, China.
FAU - Bian, S
AU  - Bian S
FAU - Li, F
AU  - Li F
FAU - Li, X F
AU  - Li XF
FAU - Fan, K
AU  - Fan K
FAU - An, H Z
AU  - An HZ
FAU - Jia, X X
AU  - Jia XX
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Collagen Type II)
RN  - 0 (Interleukins)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Allografts
MH  - Animals
MH  - *Arthritis, Experimental
MH  - Arthritis, Rheumatoid
MH  - Blotting, Western
MH  - Collagen Type II
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukins
MH  - Matrix Metalloproteinase 3
MH  - Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Rats
MH  - Synovial Membrane
MH  - Interleukin-22
OTO - NOTNLM
OT  - IL-22
OT  - MMP-3
OT  - Mesenchymal stem cells
OT  - Rheumatoid arthritis
EDAT- 2017/03/16 06:00
MHDA- 2018/09/15 06:00
CRDT- 2017/03/16 06:00
PHST- 2017/03/16 06:00 [entrez]
PHST- 2017/03/16 06:00 [pubmed]
PHST- 2018/09/15 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2017.09.014 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2017 Mar 7;97(9):698-702. doi: 
      10.3760/cma.j.issn.0376-2491.2017.09.014.