PMID- 28299336
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20220330
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2017
DP  - 2017
TI  - SHISA3 Promoter Methylation Is a Potential Diagnostic and Prognostic Biomarker 
      for Laryngeal Squamous Cell Carcinoma.
PG  - 9058749
LID - 10.1155/2017/9058749 [doi]
LID - 9058749
AB  - The purpose of this study was to evaluate the contribution of SHISA3 promoter 
      methylation to laryngeal squamous cell carcinoma (LSCC). SHISA3 promoter 
      methylation status and expression were determined using methylation-specific 
      polymerase chain reaction (MSP) and quantitative real-time PCR (qRT-PCR) in 93 
      paired LSCC and adjacent normal tissues, respectively. Furthermore, the 
      regulatory function of the SHISA3 promoter fragment was analyzed using a 
      luciferase reporter assay. The results reveal that there is a significant 
      increase in SHISA3 methylation in LSCC tissues compared with corresponding 
      nontumor tissues (P = 4.58E - 12). The qRT-PCR results show a significant 
      association between SHISA3 methylation and expression in LSCC (P = 1.67E - 03). 
      In addition, the area under the receiver operating characteristic curve was 0.91. 
      Consequently, a log-rank test and multivariate Cox analysis suggest that SHISA3 
      promoter hypermethylation is a predictor of poor overall survival for LSCC 
      (log-rank P = 0.024; HR = 2.71; 95% CI = 1.024-7.177; P = 0.047). The results 
      indicate that SHISA3 promoter hypermethylation might increase the risk of LSCC 
      through regulation of gene expression and is a potential diagnostic and 
      prognostic biomarker for LSCC.
FAU - Shen, Zhisen
AU  - Shen Z
AUID- ORCID: 0000-0001-6660-0488
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
FAU - Zhou, Chongchang
AU  - Zhou C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
FAU - Li, Jinyun
AU  - Li J
AD  - School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China.
FAU - Ye, Dong
AU  - Ye D
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China.
FAU - Cao, Bin
AU  - Cao B
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
FAU - Hao, Wenjuan
AU  - Hao W
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
FAU - Lin, Lexi
AU  - Lin L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China; School of Medicine, Ningbo 
      University, Ningbo, Zhejiang 315211, China.
FAU - Zhang, Yuna
AU  - Zhang Y
AUID- ORCID: 0000-0002-3417-0484
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital, Ningbo 
      University, Ningbo, Zhejiang 315040, China.
LA  - eng
PT  - Journal Article
DEP - 20170216
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics/mortality
MH  - *DNA Methylation
MH  - Epigenesis, Genetic
MH  - Follow-Up Studies
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Genes, Tumor Suppressor
MH  - HEK293 Cells
MH  - Head and Neck Neoplasms/diagnosis/*genetics/mortality
MH  - Humans
MH  - Laryngeal Neoplasms/diagnosis/*genetics/mortality
MH  - Membrane Proteins/*genetics
MH  - Prognosis
MH  - *Promoter Regions, Genetic
MH  - Proportional Hazards Models
MH  - ROC Curve
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Transcriptional Activation
PMC - PMC5337399
COIS- The authors declare that there is no conflict of interests regarding the 
      publication of this article.
EDAT- 2017/03/17 06:00
MHDA- 2017/04/18 06:00
PMCR- 2017/02/16
CRDT- 2017/03/17 06:00
PHST- 2016/09/04 00:00 [received]
PHST- 2016/11/03 00:00 [revised]
PHST- 2017/01/10 00:00 [accepted]
PHST- 2017/03/17 06:00 [entrez]
PHST- 2017/03/17 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2017/02/16 00:00 [pmc-release]
AID - 10.1155/2017/9058749 [doi]
PST - ppublish
SO  - Biomed Res Int. 2017;2017:9058749. doi: 10.1155/2017/9058749. Epub 2017 Feb 16.