PMID- 28302561
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20220310
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 176
DP  - 2017 May 1
TI  - Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in 
      rats.
PG  - 75-81
LID - S0024-3205(17)30092-9 [pii]
LID - 10.1016/j.lfs.2017.03.009 [doi]
AB  - AIM: Gentamicin (GEN) is an aminoglycoside antibiotic employed in treatment of 
      life-threatening gram-negative infections, but one of its major side effects is 
      the induction of nephrotoxicity. Therefore, the aim of this work was to 
      scrutinize the possible protective effect of gossypin against GEN-induced 
      nephrotoxicity. METHOD: Rats were randomly divided into four groups. First group 
      served as a control, second group was injected with gossypin (10mg/kg, orally) 
      for 7days, third group was injected with GEN (80mg/kg, i.p.) and the fourth group 
      was co-treated with GEN and gossypin for 7days. KEY FINDING: GEN-treated group 
      showed kidney dysfunction as proteinuria excretion rate, podocalyxin excretion 
      rates, renal monocyte chemoattractant protein-1 (MCP-1), blood urea nitrogen 
      (BUN) and plasma creatinine were significantly increased as well as tubular 
      degeneration occur. The significant decrease in renal reduced glutathione (GSH) 
      level, superoxide dismutase (SOD) and catalase (CAT) activities and an increase 
      in thiobarbituric acid reactive substances (TBARs) level, indicated that 
      GEN-induced nephrotoxicity through oxidative stress reactions. Also, GEN 
      up-regulated both gene expression and renal levels of inflammatory cytokines 
      TNF-alpha and IL-6. On the other hand, concurrent treatment of gossypin plus GEN 
      protected kidney tissues against nephrotoxic effects of GEN through elevated 
      levels of renal GSH, SOD and CAT activities while decreased in renal TBARs level. 
      In addition, gossypin down-regulated gene expression and renal levels of 
      inflammatory cytokines TNF-alpha and IL-6 induced by GEN. SIGNIFICANCE: This study 
      revealed that gossypin exerts protection against nephrotoxicity induced by 
      gentamicin via its antioxidant activity.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Katary, Mohamed
AU  - Katary M
AD  - Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour 
      University, Egypt.
FAU - Salahuddin, Ahmad
AU  - Salahuddin A
AD  - Biochemistry Department, Faculty of Pharmacy, Damanhour University, Egypt. 
      Electronic address: salahuddin@pharm.dmu.edu.eg.
LA  - eng
PT  - Journal Article
DEP - 20170314
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Flavonoids)
RN  - 0 (Gentamicins)
RN  - 0 (Interleukin-6)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (podocalyxin)
RN  - A3Q367XWX9 (gossypin)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Catalase/metabolism
MH  - Chemokine CCL2/metabolism
MH  - Flavonoids/*pharmacology
MH  - Gentamicins/*adverse effects/pharmacology
MH  - Interleukin-6/metabolism
MH  - *Kidney/metabolism/pathology
MH  - Male
MH  - *Proteinuria/chemically induced/metabolism/pathology/prevention & control
MH  - Rats
MH  - Sialoglycoproteins/metabolism
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Antioxidant
OT  - Gentamicin
OT  - Gossypin
OT  - IL-6
OT  - Monocyte chemoattractant protein-1
OT  - Nephrotoxicity
OT  - TNF-alpha
EDAT- 2017/03/18 06:00
MHDA- 2017/04/30 06:00
CRDT- 2017/03/18 06:00
PHST- 2016/12/29 00:00 [received]
PHST- 2017/03/08 00:00 [revised]
PHST- 2017/03/12 00:00 [accepted]
PHST- 2017/03/18 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2017/03/18 06:00 [entrez]
AID - S0024-3205(17)30092-9 [pii]
AID - 10.1016/j.lfs.2017.03.009 [doi]
PST - ppublish
SO  - Life Sci. 2017 May 1;176:75-81. doi: 10.1016/j.lfs.2017.03.009. Epub 2017 Mar 14.