PMID- 2830485
OWN - NLM
STAT- MEDLINE
DCOM- 19880401
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 7
IP  - 12
DP  - 1987 Dec
TI  - Glucocorticoid-resistant lymphoma cell variants that contain functional 
      glucocorticoid receptors.
PG  - 4211-7
AB  - A mouse T-lymphosarcoma cell line stably infected with mouse mammary tumor virus 
      (MMTV) was used as the parent line for a genetic analysis of two glucocorticoid 
      hormone responses, hormone-induced cytolysis and stimulation of viral gene 
      expression. Variants were selected for survival and elevated expression of MMTV 
      proteins in the presence of the steroid. The MMTV marker provided a sensitive 
      test for glucocorticoid receptor (GR) function in the hormone-resistant variants. 
      This strategy resulted in the isolation of two novel types of hormone-resistant 
      variants. One type of variant with only about 25% of the level of GR found in the 
      parent line was resistant to the cytolytic effects of glucocorticoid but produced 
      increased levels of MMTV gene products in response to the hormone. This variant 
      phenotype demonstrated that the MMTV response requires fewer GR than the 
      cytolytic response. Another variant, which required approximately 100-fold higher 
      concentrations of hormone than the wild-type cells for both responses, apparently 
      contained GR with altered hormone-binding properties.
FAU - Rabindran, S K
AU  - Rabindran SK
AD  - Department of Pathology, University of Southern California School of Medicine, 
      Los Angeles 90033.
FAU - Danielsen, M
AU  - Danielsen M
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
GR  - CA-01149/CA/NCI NIH HHS/United States
GR  - GM-36317/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Glucocorticoids)
RN  - 0 (Glycoproteins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Dexamethasone/metabolism/pharmacology
MH  - Drug Resistance/genetics
MH  - *Genetic Variation
MH  - Glucocorticoids/*pharmacology
MH  - Glycoproteins/biosynthesis
MH  - Lymphoma, Non-Hodgkin/*genetics/metabolism/microbiology
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mutation
MH  - RNA, Neoplasm/analysis
MH  - RNA, Viral/metabolism
MH  - Receptors, Glucocorticoid/drug effects/genetics/*physiology
MH  - *T-Lymphocytes
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - Viral Proteins/biosynthesis
PMC - PMC368102
EDAT- 1987/12/01 00:00
MHDA- 1987/12/01 00:01
PMCR- 1987/12/01
CRDT- 1987/12/01 00:00
PHST- 1987/12/01 00:00 [pubmed]
PHST- 1987/12/01 00:01 [medline]
PHST- 1987/12/01 00:00 [entrez]
PHST- 1987/12/01 00:00 [pmc-release]
AID - 10.1128/mcb.7.12.4211-4217.1987 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1987 Dec;7(12):4211-7. doi: 10.1128/mcb.7.12.4211-4217.1987.