PMID- 28306175
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20171106
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 38
IP  - 13-14
DP  - 2017 Jul
TI  - A stable and convenient protein electrophoresis titration device with bubble 
      removing system.
PG  - 1706-1712
LID - 10.1002/elps.201600472 [doi]
AB  - Moving reaction boundary titration (MRBT) has a potential application to 
      immunoassay and protein content analysis with high selectivity. However, air 
      bubbles often impair the accuracy of MRBT, and the leakage of electrolyte greatly 
      decreases the safety and convenience of electrophoretic titration. Addressing 
      these two issues a reliable MRBT device with modified electrolyte chamber of 
      protein titration was designed. Multiphysics computer simulation was conducted 
      for optimization according to two-phase flow. The single chamber was made of two 
      perpendicular cylinders with different diameters. After placing electrophoretic 
      tube, the resident air in the junction next to the gel could be eliminated by a 
      simple fast electrolyte flow. Removing the electrophoretic tube automatically 
      prevented electrolyte leakage at the junction due to the gravity-induced negative 
      pressure within the chamber. Moreover, the numerical simulation and experiments 
      showed that the improved MRBT device has following advantages: (i) easy and rapid 
      setup of electrophoretic tube within 20 s; (ii) simple and quick bubble 
      dissipates from the chamber of titration within 2 s; (iii) no electrolyte leakage 
      from the two chambers: and (iv) accurate protein titration and safe instrumental 
      operation. The developed technique and apparatus greatly improves the performance 
      of the previous MRBT device, and providing a new route toward practical 
      application.
CI  - (c) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Zhang, Qiang
AU  - Zhang Q
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Fan, Liu-Yin
AU  - Fan LY
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Li, Wen-Lin
AU  - Li WL
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Cong, Feng-Song
AU  - Cong FS
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Zhong, Ran
AU  - Zhong R
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Chen, Jing-Jing
AU  - Chen JJ
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - He, Yu-Chen
AU  - He YC
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Xiao, Hua
AU  - Xiao H
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
FAU - Cao, Cheng-Xi
AU  - Cao CX
AD  - Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of 
      Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao 
      Tong University, Shanghai, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170407
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Proteins)
SB  - IM
MH  - Computer Simulation
MH  - Electrophoresis/*instrumentation/*methods
MH  - Equipment Design
MH  - Proteins/*analysis/*chemistry
OTO - NOTNLM
OT  - Computer simulation
OT  - Flow venting
OT  - Moving reaction boundary electrophoresis
OT  - Protein titration
EDAT- 2017/03/18 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/03/18 06:00
PHST- 2016/06/16 00:00 [received]
PHST- 2017/03/02 00:00 [revised]
PHST- 2017/03/09 00:00 [accepted]
PHST- 2017/03/18 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/03/18 06:00 [entrez]
AID - 10.1002/elps.201600472 [doi]
PST - ppublish
SO  - Electrophoresis. 2017 Jul;38(13-14):1706-1712. doi: 10.1002/elps.201600472. Epub 
      2017 Apr 7.