PMID- 28306606
OWN - NLM
STAT- MEDLINE
DCOM- 20180110
LR  - 20180110
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 28
IP  - 6
DP  - 2017 Apr 12
TI  - Spinal microglial P2X4 receptor-brain-derived neurotrophic factor signaling 
      regulates nicotine withdrawal-induced hyperalgesia.
PG  - 339-347
LID - 10.1097/WNR.0000000000000769 [doi]
AB  - Nicotine withdrawal (NTW) has been shown to increase pain sensitivity. However, 
      the pathogenesis of NTW-induced hyperalgesia syndrome is unknown. Microglial 
      activation, with increased expression of the P2X4 receptor (P2X4R) and 
      brain-derived neurotrophic factor (BDNF) as important markers, is associated with 
      hyperalgesia; therefore, these markers may represent an unprecedented target to 
      prevent hyperalgesia. In this study, we explored the contributions of spinal 
      microglial P2X4R-BDNF signaling in NTW-induced hyperalgesia. Immunohistochemical 
      analysis showed that spinal microglia were activated and that the P2X4R level was 
      increased and colocalized with ionized calcium-binding adapter molecule 1 in 
      NTW-induced hyperalgesia. Furthermore, we showed that microglial activation with 
      NTW resulted in an increased expression of spinal P2X4R and an elevated release 
      of BDNF. Intrathecal minocycline (a specific inhibitor of microglial activation) 
      reversed thermal hyperalgesia as well as increased the spinal microglial P2X4R 
      and BDNF levels induced by NTW. To the best of our knowledge, the present study 
      provides evidence that spinal microglial P2X4R-BDNF signaling is critical for the 
      development of NTW-induced hyperalgesia.
FAU - Zhang, Xiaodi
AU  - Zhang X
AD  - aJiangsu Province Key Laboratory of Anesthesiology, Jiangsu Province Key 
      Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical 
      University, Xuzhou, Jiangsu Province bDepartment of Anesthesiology, Liaocheng 
      People's Hospital, Liaocheng, Shandong cDepartment of Medicine, Hebei North 
      University, Zhangjiakou, Hebei, China.
FAU - Xu, Pengcheng
AU  - Xu P
FAU - Li, Chengbao
AU  - Li C
FAU - Zhu, Wenchao
AU  - Zhu W
FAU - Wu, Shanshan
AU  - Wu S
FAU - Yu, Ailan
AU  - Yu A
FAU - Ding, Yonghong
AU  - Ding Y
FAU - Wang, Qinghe
AU  - Wang Q
FAU - Zhang, Zongwang
AU  - Zhang Z
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Aif1 protein, rat)
RN  - 0 (Analgesics, Non-Narcotic)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Receptors, Purinergic P2X4)
RN  - 6M3C89ZY6R (Nicotine)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Analgesics, Non-Narcotic/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Disease Models, Animal
MH  - Hot Temperature
MH  - Hyperalgesia/chemically induced/drug therapy/*metabolism/pathology
MH  - Male
MH  - Microfilament Proteins/metabolism
MH  - Microglia/drug effects/*metabolism/pathology
MH  - Minocycline/pharmacology
MH  - Nicotine/toxicity
MH  - Nicotinic Agonists/toxicity
MH  - Pain Threshold/drug effects/physiology
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X4/*metabolism
MH  - Spinal Cord/drug effects/*metabolism/pathology
MH  - Substance Withdrawal Syndrome/drug therapy/*metabolism/pathology
EDAT- 2017/03/18 06:00
MHDA- 2018/01/11 06:00
CRDT- 2017/03/18 06:00
PHST- 2017/03/18 06:00 [pubmed]
PHST- 2018/01/11 06:00 [medline]
PHST- 2017/03/18 06:00 [entrez]
AID - 10.1097/WNR.0000000000000769 [doi]
PST - ppublish
SO  - Neuroreport. 2017 Apr 12;28(6):339-347. doi: 10.1097/WNR.0000000000000769.