PMID- 28314114
OWN - NLM
STAT- MEDLINE
DCOM- 20180322
LR  - 20220318
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 60
IP  - 7
DP  - 2017 Jul
TI  - St(2)-80: a new FISH marker for St genome and genome analysis in Triticeae.
PG  - 553-563
LID - 10.1139/gen-2016-0228 [doi]
AB  - The St genome is one of the most fundamental genomes in Triticeae. Repetitive 
      sequences are widely used to distinguish different genomes or species. The 
      primary objectives of this study were to (i) screen a new sequence that could 
      easily distinguish the chromosome of the St genome from those of other genomes by 
      fluorescence in situ hybridization (FISH) and (ii) investigate the genome 
      constitution of some species that remain uncertain and controversial. We used 
      degenerated oligonucleotide primer PCR (Dop-PCR), Dot-blot, and FISH to screen 
      for a new marker of the St genome and to test the efficiency of this marker in 
      the detection of the St chromosome at different ploidy levels. Signals produced 
      by a new FISH marker (denoted St(2)-80) were present on the entire arm of 
      chromosomes of the St genome, except in the centromeric region. On the contrary, 
      St(2)-80 signals were present in the terminal region of chromosomes of the E, H, 
      P, and Y genomes. No signal was detected in the A and B genomes, and only weak 
      signals were detected in the terminal region of chromosomes of the D genome. 
      St(2)-80 signals were obvious and stable in chromosomes of different genomes, 
      whether diploid or polyploid. Therefore, St(2)-80 is a potential and useful FISH 
      marker that can be used to distinguish the St genome from those of other genomes 
      in Triticeae.
FAU - Wang, Long
AU  - Wang L
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Shi, Qinghua
AU  - Shi Q
AD  - b State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, 
      China.
FAU - Su, Handong
AU  - Su H
AD  - b State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, 
      China.
FAU - Wang, Yi
AU  - Wang Y
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Sha, Lina
AU  - Sha L
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Fan, Xing
AU  - Fan X
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Kang, Houyang
AU  - Kang H
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Zhang, Haiqin
AU  - Zhang H
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
FAU - Zhou, Yonghong
AU  - Zhou Y
AD  - a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, 
      Chengdu, Sichuan, China.
AD  - c Key Laboratory of Genetic Resources and Crop Improvement, Ministry of 
      Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170317
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - *Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Ploidies
MH  - Poaceae/classification/*genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
OTO - NOTNLM
OT  - Elytrigia sensu lato
OT  - St2-80
OT  - composition genomique
OT  - genome constitution
OT  - marqueur special
OT  - repetitive sequences
OT  - special marker
OT  - sequences repetees
EDAT- 2017/03/18 06:00
MHDA- 2018/03/23 06:00
CRDT- 2017/03/18 06:00
PHST- 2017/03/18 06:00 [pubmed]
PHST- 2018/03/23 06:00 [medline]
PHST- 2017/03/18 06:00 [entrez]
AID - 10.1139/gen-2016-0228 [doi]
PST - ppublish
SO  - Genome. 2017 Jul;60(7):553-563. doi: 10.1139/gen-2016-0228. Epub 2017 Mar 17.