PMID- 28315324
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20231213
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 486
IP  - 2
DP  - 2017 Apr 29
TI  - Nucleolin protects macrophages from oxLDL-induced foam cell formation through 
      up-regulating ABCA1 expression.
PG  - 364-371
LID - S0006-291X(17)30505-3 [pii]
LID - 10.1016/j.bbrc.2017.03.047 [doi]
AB  - Our recent studies have indicated that nucleolin, as a multifunctional 
      RNA-binding protein, exerts protective effects in the myocardial cells and 
      endothelial cells under the condition of oxidative stress. However, the function 
      of nucleolin and its potential mechanism in macrophage-derived foam cell 
      formation remain largely unexplored. ApoE-/- mice were fed with a high-fat diet 
      (HFD) for 10-24 weeks. Protein expression was measured by western blotting or 
      immunofluorescence, and gene expression at the mRNA level was detected by 
      qRT-PCR. The level of lipid in macrophages was examined by Oil Red O staining, 
      high-performance liquid chromatography (HPLC) and NBD-cholesterol. Actinomycin D 
      (Act D) was used to determine the stability of ABCA1 mRNA in macrophages. The 
      interaction of nucleolin with ABCA1 mRNA was assessed using 
      co-immunoprecipitation (co-IP). The aortas advanced plaques demonstrated 
      significantly lower levels of nucleolin protein compared with early plaques in 
      ApoE-/- mice, in which the macrophage foam cells occupied main body. Nucleolin 
      expression at the mRNA and protein levels in RAW264.7 macrophages was 
      significantly reduced by oxidized low-density lipoprotein (oxLDL) in a dose- and 
      time-dependent manner. Furthermore, nucleolin overexpression markedly attenuated 
      lipid accumulation in oxLDL-challenged macrophages through increasing cholesterol 
      efflux. In addition, nucleolin overexpression significantly increased the 
      expression of ATP-binding cassette transporter A1 (ABCA1) at the mRNA and protein 
      levels without affecting expressions of scavenger receptors (SR)-A, SR-B1, CD36 
      and ATP-binding cassette transporter G1 (ABCG1) at the mRNA level. Moreover, 
      nucleolin overexpression increased the stability of ABCA1 mRNA in macrophages, 
      whereas nucleolin ablation abrogated the oxLDL-induced up-regulation of ABCA1. 
      The up-regulation of ABCA1 by nucleolin resulted from its protein-RNA 
      interaction. Our data suggested that nucleolin inhibited foam cell formation 
      through enhancing stability of ABCA1 mRNA and subsequently increasing cholesterol 
      efflux.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Li, Yuanbin
AU  - Li Y
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Jiang, Bimei
AU  - Jiang B
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China. Electronic address: 
      jiangbimei@163.com.
FAU - Liang, Pengfei
AU  - Liang P
AD  - Department of Burns and Plastic Surgery, Xiangya Hospital, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Tong, Zhongyi
AU  - Tong Z
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Liu, Meidong
AU  - Liu M
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Lv, Qinglan
AU  - Lv Q
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Liu, Yanjuan
AU  - Liu Y
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Liu, Xuanyou
AU  - Liu X
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Tang, Yuting
AU  - Tang Y
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
FAU - Xiao, Xianzhong
AU  - Xiao X
AD  - Department of Pathophysiology, Xiangya School of Medicine, Central South 
      University, Changsha, Hunan 410008, PR China.
LA  - eng
PT  - Journal Article
DEP - 20170314
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ABCA1 protein, mouse)
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (Apolipoproteins E)
RN  - 0 (CD36 Antigens)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Scarb1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class A)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (oxidized low density lipoprotein)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/*genetics/metabolism
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1/genetics/metabolism
MH  - Animals
MH  - Apolipoproteins E/deficiency/genetics
MH  - Atherosclerosis/etiology/*genetics/metabolism/pathology
MH  - Biological Transport/drug effects
MH  - CD36 Antigens/genetics/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cholesterol/metabolism
MH  - Diet, High-Fat
MH  - Dose-Response Relationship, Drug
MH  - Foam Cells/drug effects/metabolism/pathology
MH  - Gene Expression Regulation
MH  - Hyperlipidemias/etiology/*genetics/metabolism/pathology
MH  - Lipoproteins, LDL/*pharmacology
MH  - Macrophages/drug effects/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Phosphoproteins/*genetics/metabolism
MH  - RNA Stability
MH  - RNA, Messenger/*genetics/metabolism
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Scavenger Receptors, Class A/genetics/metabolism
MH  - Scavenger Receptors, Class B/genetics/metabolism
MH  - Signal Transduction
MH  - Nucleolin
OTO - NOTNLM
OT  - ABCA1
OT  - Atherosclerosis
OT  - Lipoprotein
OT  - Nucleolin
OT  - mRNA stability
EDAT- 2017/03/21 06:00
MHDA- 2017/06/06 06:00
CRDT- 2017/03/19 06:00
PHST- 2017/02/24 00:00 [received]
PHST- 2017/03/13 00:00 [accepted]
PHST- 2017/03/21 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2017/03/19 06:00 [entrez]
AID - S0006-291X(17)30505-3 [pii]
AID - 10.1016/j.bbrc.2017.03.047 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Apr 29;486(2):364-371. doi: 
      10.1016/j.bbrc.2017.03.047. Epub 2017 Mar 14.