PMID- 28316373 OWN - NLM STAT- MEDLINE DCOM- 20170710 LR - 20181113 IS - 1466-1861 (Electronic) IS - 0962-9351 (Print) IS - 0962-9351 (Linking) VI - 2017 DP - 2017 TI - Nuclear Factor of Activated T Cells and Cytokines Gene Expression of the T Cells in AIDS Patients with Immune Reconstitution Inflammatory Syndrome during Highly Active Antiretroviral Therapy. PG - 1754741 LID - 10.1155/2017/1754741 [doi] LID - 1754741 AB - Background. The etiology of immune reconstitution inflammatory syndrome (IRIS) in AIDS patients after the initiation of HAART remains unknown. Several researches indicated that the development of IRIS is associated with the production and variation of cytokines, whose gene expression are closely related to the Ca2(+)/CN-nuclear factor of activated T cells (NFAT) pathway. Methods. We studied the expression of NFAT isoforms and their major target cytokines genes in peripheral blood CD3(+) T cells of subjects through fluorescence quantitative PCR and explored the expression changes of these genes before and after HAART. Results. After the initiation of HARRT, NFAT1, IL-6, and IL-8 gene expression showed a reversal trend in the CD3(+) T cells of the IRIS group and changed from low expression before HARRT to high expression after HARRT. In particular, the relative gene expression of NFAT1 was markedly higher compared with the other three isoforms. The IRIS group also showed higher NFAT4, NFAT2, NFAT1, IL-1beta, IL-10, IL-2, IL-18, and TNF-alpha gene expression than the non-IRIS group. Conclusion. This study suggested that high expression levels of IL-2, IL-6, IL-8, TNF-alpha, IL-1beta, IL-10, IL-12, and IL-18 can predict the risk of IRIS. The increased expression of NFAT1 and NFAT4 may promote the expression of cytokines, such as IL-6, IL-8, and TNF-alpha, which may promote the occurrence of IRIS. FAU - Sun, Jia AU - Sun J AUID- ORCID: 0000-0002-9683-1065 AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Chen, Heling AU - Chen H AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Xie, Yirui AU - Xie Y AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Su, Junwei AU - Su J AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Huang, Ying AU - Huang Y AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Xu, Lijun AU - Xu L AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Yin, Michael AU - Yin M AD - Division of Infectious Diseases, Columbia University Medical Center, New York, NY 10032, USA. FAU - Zhou, Qihui AU - Zhou Q AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. FAU - Zhu, Biao AU - Zhu B AUID- ORCID: 0000-0001-6288-4575 AD - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. LA - eng PT - Journal Article DEP - 20170220 PL - United States TA - Mediators Inflamm JT - Mediators of inflammation JID - 9209001 RN - 0 (CD3 Complex) RN - 0 (Cytokines) RN - 0 (Interleukin-18) RN - 0 (Interleukin-1beta) RN - 0 (Interleukin-6) RN - 0 (Interleukin-8) RN - 0 (NFATC Transcription Factors) RN - 0 (Tumor Necrosis Factor-alpha) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Acquired Immunodeficiency Syndrome/drug therapy/*genetics MH - Adult MH - *Antiretroviral Therapy, Highly Active MH - CD3 Complex/genetics MH - Cytokines/*genetics MH - Female MH - Humans MH - Immune Reconstitution Inflammatory Syndrome/*genetics MH - Interleukin-10/genetics MH - Interleukin-18/genetics MH - Interleukin-1beta/genetics MH - Interleukin-6/genetics MH - Interleukin-8/genetics MH - Male MH - NFATC Transcription Factors/*genetics MH - Reverse Transcriptase Polymerase Chain Reaction MH - T-Lymphocytes/*metabolism MH - Tumor Necrosis Factor-alpha/genetics PMC - PMC5337872 COIS- All authors declare that they have no potential competing interests. EDAT- 2017/03/21 06:00 MHDA- 2017/07/14 06:00 PMCR- 2017/02/20 CRDT- 2017/03/21 06:00 PHST- 2016/10/27 00:00 [received] PHST- 2017/01/24 00:00 [accepted] PHST- 2017/03/21 06:00 [entrez] PHST- 2017/03/21 06:00 [pubmed] PHST- 2017/07/14 06:00 [medline] PHST- 2017/02/20 00:00 [pmc-release] AID - 10.1155/2017/1754741 [doi] PST - ppublish SO - Mediators Inflamm. 2017;2017:1754741. doi: 10.1155/2017/1754741. Epub 2017 Feb 20.