PMID- 28318412
OWN - NLM
STAT- MEDLINE
DCOM- 20171102
LR  - 20180116
IS  - 1557-8542 (Electronic)
IS  - 1545-8547 (Linking)
VI  - 14
IP  - 3
DP  - 2017 Jun
TI  - Effects of Lithium and 2,4-Dichlorophenol on Zebrafish: Circadian Rhythm Disorder 
      and Molecular Effects.
PG  - 209-215
LID - 10.1089/zeb.2016.1389 [doi]
AB  - The aim of this study was to investigate lithium and 2,4-dichlorophenol 
      (2,4-DCP)-induced circadian rhythm disorder and their genome-wide effects in 
      zebrafish. Zebrafish larvae were exposed to 250 ppm LiCl (n = 40) or 20 ppm 
      2,4-DCP. RNA was subsequently extracted and determined quantitatively. The mRNA 
      levels of circadian clock-related genes, including clock1a, bmal1b, per2, and 
      per1b, were determined. Microarray datasets were generated and the differentially 
      expressed genes (DEGs) were identified. The mRNA levels of some upregulated and 
      downregulated DEGs were examined by quantitative real-time polymerase chain 
      reaction (RT-PCR). Finally, gene ontology (GO) enrichment analysis was applied to 
      determine the roles of the DEGs. The mRNA expression levels of circadian 
      rhythm-related genes in the daily cycle were significantly affected after 
      incubation of zebrafish with LiCl and 2,4-DCP. Many genes were differentially 
      expressed during the light phase (97 h) and RT-PCR validation tests revealed that 
      the expression patterns of DEGs were in accordance with those obtained by 
      microarray analysis. GO functional enrichment analysis showed that the DEGs in 
      LiCl- and 2,4-DCP-treated groups were associated with signal transduction and 
      development. Collectively, our findings indicate that LiCl and 2,4-DCP could 
      affect signal transduction pathways and immune response, thereby inducing 
      circadian rhythm disorder.
FAU - Xiao, Bo
AU  - Xiao B
AD  - 1 Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of 
      Environmental Science and Engineering, Yancheng Institute of Technology , 
      Yancheng, Jiangsu, China .
FAU - Cui, Li-Qiang
AU  - Cui LQ
AD  - 1 Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of 
      Environmental Science and Engineering, Yancheng Institute of Technology , 
      Yancheng, Jiangsu, China .
FAU - Ding, Cheng
AU  - Ding C
AD  - 1 Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of 
      Environmental Science and Engineering, Yancheng Institute of Technology , 
      Yancheng, Jiangsu, China .
FAU - Wang, Han
AU  - Wang H
AD  - 2 School of Biology and Basic Medical Sciences, Medical College, Soochow 
      University , Suzhou, Jiangsu, China .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170320
PL  - United States
TA  - Zebrafish
JT  - Zebrafish
JID - 101225070
RN  - 0 (Anthelmintics)
RN  - 0 (Chlorophenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants)
RN  - 0 (Zebrafish Proteins)
RN  - 9FN79X2M3F (Lithium)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Animals
MH  - Anthelmintics/pharmacology
MH  - Chlorophenols/*toxicity
MH  - Chronobiology Disorders/*genetics/pathology
MH  - Circadian Rhythm/*drug effects
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Gene Ontology
MH  - Larva/drug effects/metabolism
MH  - Lithium/*toxicity
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction/drug effects
MH  - Water Pollutants/*toxicity
MH  - Zebrafish/growth & development/*physiology
MH  - Zebrafish Proteins/genetics/metabolism
OTO - NOTNLM
OT  - 2,4-dichlorophenol
OT  - circadian rhythms
OT  - lithium
OT  - zebrafish
EDAT- 2017/03/21 06:00
MHDA- 2017/11/03 06:00
CRDT- 2017/03/21 06:00
PHST- 2017/03/21 06:00 [pubmed]
PHST- 2017/11/03 06:00 [medline]
PHST- 2017/03/21 06:00 [entrez]
AID - 10.1089/zeb.2016.1389 [doi]
PST - ppublish
SO  - Zebrafish. 2017 Jun;14(3):209-215. doi: 10.1089/zeb.2016.1389. Epub 2017 Mar 20.