PMID- 28318643 OWN - NLM STAT- MEDLINE DCOM- 20170717 LR - 20210103 IS - 1095-6859 (Electronic) IS - 0090-8258 (Linking) VI - 145 IP - 3 DP - 2017 Jun TI - CXCL10 alters the tumour immune microenvironment and disease progression in a syngeneic murine model of high-grade serous ovarian cancer. PG - 436-445 LID - S0090-8258(17)30208-1 [pii] LID - 10.1016/j.ygyno.2017.03.007 [doi] AB - OBJECTIVE: We recently established that high STAT1 expression and associated T helper type I tumour immune microenvironment (TME) are prognostic and chemotherapy response predictive biomarkers in high-grade serous ovarian cancer (HGSC). STAT1 induced chemokine CXCL10 is key to the recruitment of lymphocytes in the TME and is significantly highly expressed in the tumours from patients with longer survival. In the current study we therefore aimed to elucidate the role CXCL10 in disease progression and tumour immune transcriptomic alterations using the ID8 syngeneic murine model of HGSC. METHODS: ID8 ovarian cancer cells were engineered for stable knockdown (KD) and overexpression (OX) of CXCL10. The OX and KD cell line derivatives, along with their respective vector controls, were implanted in immunocompetent C57BL/6 mice via intra-peritoneal injections. At end point, immune transcriptomic profiling of tumour tissues and multiplex cytokine profiling of ascites, was performed. Effect of CXCL10 expression on the tumour vasculature and tumour cell proliferation was evaluated by CD31 and Ki67 immunostaining, respectively. RESULTS: Increased CXCL10 expression led to decreased tumour burden and malignant ascites accumulation in the ID8 syngeneic murine model of HGSC. The ascites levels of IL-6 and VEGF were significantly reduced in OX mice compared to the vector controls. The OX tumours also showed reduced vasculature (CD31) and proliferative index (Ki67) compared to the control tumours. Significantly higher expression of genes associated with antigen processing, apoptosis and T cell function was observed in OX tumours compared to the controls. Reduced CXCL10 expression in tumours from KD mice led to increased ascites accumulation and disease progression compared to the controls. CONCLUSION: CXCL10 is a positive determinant of anti-tumour immune responses in HGSC TME and disease progression. These findings are foundational for future translational studies aimed at improving treatment response and survival in HGSC patients, via exploiting the TME. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - K Au, Katrina AU - K Au K AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Peterson, Nichole AU - Peterson N AD - Department of Obstetrics and Gynecology, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Truesdell, Peter AU - Truesdell P AD - Cancer Biology and Genetics Division, Queen's Cancer Research Institute, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Reid-Schachter, Gillian AU - Reid-Schachter G AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Khalaj, Kasra AU - Khalaj K AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Ren, Runhan AU - Ren R AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Francis, Julie-Ann AU - Francis JA AD - Department of Obstetrics and Gynecology, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Graham, Charles H AU - Graham CH AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Craig, Andrew W AU - Craig AW AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada; Cancer Biology and Genetics Division, Queen's Cancer Research Institute, Queen's University, Kingston, Ontario K7L 3N6, Canada. FAU - Koti, Madhuri AU - Koti M AD - Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada; Department of Obstetrics and Gynecology, Queen's University, Kingston, Ontario K7L 3N6, Canada; Cancer Biology and Genetics Division, Queen's Cancer Research Institute, Queen's University, Kingston, Ontario K7L 3N6, Canada. Electronic address: kotim@queensu.ca. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170317 PL - United States TA - Gynecol Oncol JT - Gynecologic oncology JID - 0365304 RN - 0 (Chemokine CXCL10) RN - 0 (Cxcl10 protein, mouse) SB - IM MH - Animals MH - Cell Line, Tumor MH - Cell Movement/immunology MH - Chemokine CXCL10/biosynthesis/genetics/*immunology MH - Cystadenocarcinoma, Serous/blood supply/genetics/*immunology/pathology MH - Disease Progression MH - Female MH - Gene Knockdown Techniques MH - Mice MH - Mice, Inbred C57BL MH - Neoplasm Grading MH - Neovascularization, Pathologic/immunology/pathology MH - Ovarian Neoplasms/blood supply/genetics/*immunology/pathology MH - Transcriptome MH - Tumor Microenvironment/*immunology OTO - NOTNLM OT - CXCL10 OT - ID8 OT - Interferon OT - Ovarian cancer OT - Tumour immune microenvironment EDAT- 2017/03/21 06:00 MHDA- 2017/07/18 06:00 CRDT- 2017/03/21 06:00 PHST- 2017/01/03 00:00 [received] PHST- 2017/03/03 00:00 [revised] PHST- 2017/03/10 00:00 [accepted] PHST- 2017/03/21 06:00 [pubmed] PHST- 2017/07/18 06:00 [medline] PHST- 2017/03/21 06:00 [entrez] AID - S0090-8258(17)30208-1 [pii] AID - 10.1016/j.ygyno.2017.03.007 [doi] PST - ppublish SO - Gynecol Oncol. 2017 Jun;145(3):436-445. doi: 10.1016/j.ygyno.2017.03.007. Epub 2017 Mar 17.