PMID- 2831874
OWN - NLM
STAT- MEDLINE
DCOM- 19880413
LR  - 20081121
IS  - 0391-7258 (Print)
IS  - 0391-7258 (Linking)
VI  - 31
IP  - 4
DP  - 1987
TI  - Flow cytometric evaluation of DNA digestion with S1 nuclease.
PG  - 511-20
AB  - The digestion of DNA with the S1 nuclease has been investigated by flow cytometry 
      on two cultured cell lines and on splenic lymphocytes. The nucleolytic activity 
      of the enzyme has been determined by measuring the decrease in fluorescence 
      intensity of DNA stained with Propidium Iodide (PI). The reaction kinetics could 
      be estimated through the shift of the G1 peak, since the shape of the DNA 
      histograms obtained from cell submitted to S1 digestion did not show any 
      particular variation as compared with the histograms of undigested control cells. 
      DNA digestion was found to be more pronounced when cells were previously damaged 
      with agents known to induce strand breaks, such as gamma-rays or Adriamycin. The 
      assessment of DNA sensitivity to S1 nuclease with flow cytometry may thus provide 
      a fast and reliable procedure for evaluating chromatin structural changes, both 
      in physiological and pathological conditions.
FAU - Prosperi, E
AU  - Prosperi E
AD  - Dipartimento di Biologia Animale dell'Universita, Pavia, Italy.
FAU - Bottiroli, G
AU  - Bottiroli G
FAU - Supino, R
AU  - Supino R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Basic Appl Histochem
JT  - Basic and applied histochemistry
JID - 7910664
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.1.- (Endonucleases)
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleus/drug effects/*metabolism
MH  - DNA, Neoplasm/drug effects/*metabolism/radiation effects
MH  - Endonucleases/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Lymphocytes/drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nucleic Acid Denaturation/*drug effects
MH  - Single-Strand Specific DNA and RNA Endonucleases
MH  - Spleen/cytology/drug effects
MH  - Time Factors
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - Basic Appl Histochem. 1987;31(4):511-20.