PMID- 28322800
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20191008
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 552
DP  - 2018 Jul 1
TI  - Single molecule mtDNA fiber FISH for analyzing numtogenesis.
PG  - 45-49
LID - S0003-2697(17)30131-8 [pii]
LID - 10.1016/j.ab.2017.03.015 [doi]
AB  - Somatic human cells contain thousands of copies of mitochondrial DNA (mtDNA). In 
      eukaryotes, natural transfer of mtDNA into the nucleus generates nuclear 
      mitochondrial DNA (NUMT) copies. We name this phenomenon as "numtogenesis". 
      Numtogenesis is a well-established evolutionary process reported in various 
      sequenced eukaryotic genomes. We have established a molecular tool to rapidly 
      detect and analyze NUMT insertions in whole genomes. To date, NUMT analyses 
      depend on deep genome sequencing combined with comprehensive computational 
      analyses of the whole genome. This is time consuming, cumbersome and cost 
      prohibitive. Further, most laboratories cannot accomplish such analyses due to 
      limited skills. We report the development of single-molecule mtFIBER FISH 
      (fluorescence in situ hybridization) to study numtogenesis. The development of 
      mtFIBER FISH should aid in establishing a role for numtogenesis in cancers and 
      other human diseases. This novel technique should help distinguish and monitor 
      cancer stages and progression, aid in elucidation of basic mechanisms underlying 
      tumorigenesis and facilitate analyses of processes related to early detection of 
      cancer, screening and/or cancer risk assessment.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Koo, Dal-Hoe
AU  - Koo DH
AD  - Wheat Genetics Resources Center, Department of Plant Pathology, Throckmorton 
      Plant Sciences Center, Kansas State University, Manhattan, KS 66506, United 
      States.
FAU - Singh, Bhupendra
AU  - Singh B
AD  - Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 
      35294, United States.
FAU - Jiang, Jiming
AU  - Jiang J
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, 
      United States.
FAU - Friebe, Bernd
AU  - Friebe B
AD  - Wheat Genetics Resources Center, Department of Plant Pathology, Throckmorton 
      Plant Sciences Center, Kansas State University, Manhattan, KS 66506, United 
      States.
FAU - Gill, Bikarm S
AU  - Gill BS
AD  - Wheat Genetics Resources Center, Department of Plant Pathology, Throckmorton 
      Plant Sciences Center, Kansas State University, Manhattan, KS 66506, United 
      States.
FAU - Chastain, Paul D
AU  - Chastain PD
AD  - College of Osteopathic Medicine, William Carey University, Hattiesburg, MS, 
      United States.
FAU - Manne, Upender
AU  - Manne U
AD  - Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 
      35294, United States.
FAU - Tiwari, Hemant K
AU  - Tiwari HK
AD  - Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL 
      35294, United States.
FAU - Singh, Keshav K
AU  - Singh KK
AD  - Departments of Genetics, Pathology, Environmental Health, Center for Free Radical 
      Biology, Center for Aging, UAB Comprehensive Cancer Center, University of Alabama 
      at Birmingham, AL 35294, United States; Birmingham Veterans Affairs Medical 
      Center, Birmingham, AL 35294, United States. Electronic address: kksingh@uab.edu.
LA  - eng
GR  - I01 BX001716/BX/BLRD VA/United States
GR  - R01 CA204430/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170318
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (DNA, Mitochondrial)
MH  - Biological Transport
MH  - Cell Line, Tumor
MH  - Cell Nucleus/genetics/*metabolism
MH  - DNA, Mitochondrial/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
PMC - PMC5814351
MID - NIHMS941510
EDAT- 2017/03/23 06:00
MHDA- 2019/07/31 06:00
PMCR- 2018/07/01
CRDT- 2017/03/22 06:00
PHST- 2017/02/01 00:00 [received]
PHST- 2017/03/15 00:00 [revised]
PHST- 2017/03/16 00:00 [accepted]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2019/07/31 06:00 [medline]
PHST- 2017/03/22 06:00 [entrez]
PHST- 2018/07/01 00:00 [pmc-release]
AID - S0003-2697(17)30131-8 [pii]
AID - 10.1016/j.ab.2017.03.015 [doi]
PST - ppublish
SO  - Anal Biochem. 2018 Jul 1;552:45-49. doi: 10.1016/j.ab.2017.03.015. Epub 2017 Mar 
      18.