PMID- 28324006
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20190418
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 158
IP  - 4
DP  - 2017 Apr 1
TI  - Effects of Season and Estradiol on KNDy Neuron Peptides, Colocalization With D2 
      Dopamine Receptors, and Dopaminergic Inputs in the Ewe.
PG  - 831-841
LID - 10.1210/en.2016-1830 [doi]
AB  - Seasonal reproduction in sheep is primarily due to a dramatic increase in the 
      ability of estradiol (E2) to inhibit the pulsatile secretion of 
      gonadotropin-releasing hormone (GnRH) during the nonbreeding season [anestrus 
      (ANS)]. Recent findings suggest that kisspeptin/neurokinin B/dynorphin (KNDy) 
      neurons of the arcuate nucleus (ARC) play a key role in conveying this negative 
      feedback influence, with dopaminergic projections from the retrochiasmatic area 
      acting upon KNDy cells to decrease kisspeptin release and thus inhibit GnRH 
      pulses. However, several questions remain unanswered: (1) Are the coexpressed 
      KNDy peptides, neurokinin B (NKB) and dynorphin, under seasonal regulation 
      similar to kisspeptin? (2) Are seasonal changes in these peptides and their 
      colocalization of D2 dopamine receptors (D2Rs) steroid dependent? and (3) Do KNDy 
      neurons receive direct input from dopaminergic terminals? We used dual- and 
      triple-label immunofluorescence to analyze brain sections through the ARC of 
      ovariectomized (OVX) and OVX plus E2 ewes perfused during either the breeding 
      season or ANS. Results showed (1) steroid-dependent and steroid-independent 
      seasonal changes in kisspeptin and NKB, but not dynorphin, immunoreactivity; (2) 
      increased D2R coexpression during ANS that was dependent on the presence of E2; 
      and (3) evidence that KNDy cells receive direct contact from dopaminergic 
      terminals and that this input increases during ANS. These results support the 
      hypothesis that dopamine acts to inhibit GnRH secretion in ANS by directly 
      suppressing the activity of ARC KNDy neurons, and implicate NKB as well as 
      kisspeptin in seasonal shifts in E2-negative feedback in the sheep.
CI  - Copyright (c) 2017 Endocrine Society.
FAU - Weems, Peyton
AU  - Weems P
AD  - Neuroscience Graduate Program, University of Mississippi Medical Center, 2500 
      North State Street, Jackson, MS, USA.
FAU - Smith, Jeremy
AU  - Smith J
AD  - School of Anatomy, Physiology and Human Biology, The University of Western 
      Australia, Nedlands, Western Australia, Australia.
FAU - Clarke, Iain J
AU  - Clarke IJ
AD  - Department of Physiology, Monash University, Clayton, Victoria, Australia.
FAU - Coolen, Lique M
AU  - Coolen LM
AD  - Departments of Physiology and Biophysics, University of Mississippi Medical 
      Center, 2500 North State Street, Jackson, MS, USA.
FAU - Goodman, Robert L
AU  - Goodman RL
AD  - Department of Physiology and Pharmacology, West Virginia University, Morgantown, 
      West Virginia.
FAU - Lehman, Michael N
AU  - Lehman MN
AD  - Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, 
      Jackson, Mississippi.
LA  - eng
GR  - R01 HD017864/HD/NICHD NIH HHS/United States
GR  - R01 HD039916/HD/NICHD NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, Dopamine D2)
RN  - 4TI98Z838E (Estradiol)
RN  - 74913-18-1 (Dynorphins)
RN  - 86933-75-7 (Neurokinin B)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Dopaminergic Neurons/*metabolism
MH  - Dynorphins/*metabolism
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Kisspeptins/*metabolism
MH  - Neurokinin B/*metabolism
MH  - Neurons/*drug effects/metabolism
MH  - Ovariectomy
MH  - Receptors, Dopamine D2/*metabolism
MH  - Reproduction/drug effects/physiology
MH  - Seasons
MH  - Sheep
PMC - PMC5460800
EDAT- 2017/03/23 06:00
MHDA- 2017/04/30 06:00
PMCR- 2018/04/01
CRDT- 2017/03/22 06:00
PHST- 2016/11/09 00:00 [received]
PHST- 2017/01/11 00:00 [accepted]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2017/03/22 06:00 [entrez]
PHST- 2018/04/01 00:00 [pmc-release]
AID - 2908844 [pii]
AID - endo_161830 [pii]
AID - 10.1210/en.2016-1830 [doi]
PST - ppublish
SO  - Endocrinology. 2017 Apr 1;158(4):831-841. doi: 10.1210/en.2016-1830.