PMID- 2832431
OWN - NLM
STAT- MEDLINE
DCOM- 19880422
LR  - 20190828
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 18
IP  - 4
DP  - 1987 Dec
TI  - Purification of envelope glycoproteins of human T cell lymphotropic virus type I 
      (HTLV-I) by affinity chromatography.
PG  - 243-55
AB  - The external envelope glycoprotein (gp46) and transmembrane glycoprotein (gp21) 
      of human T-cell lymphotropic virus type I (HTLV-I) were isolated from lysates of 
      HTLV-I-infected HUT-102 cells by affinity chromatography. Fifty ml aliquots of 
      packed HUT-102 cells were extracted with 1% Triton X-100, and lysates were 
      treated sequentially with an affinity column containing IgG from an HTLV-I+ human 
      subject followed by chromatography of the bound fraction over a lentil lectin 
      column. The identity of the purified envelope proteins was confirmed with a human 
      monoclonal antibody (0.5 alpha) to gp46 and with rabbit antisera raised to a 
      synthetic peptide from the C-terminus of gp21. Affinity-purified envelope 
      glycoproteins were bound to microtiter wells and used in radioimmunoassay to 
      detect murine and human anti-envelope antibodies to gp46 and gp21 molecules.
FAU - Palker, T J
AU  - Palker TJ
AD  - Department of Medicine, Duke University Medical Center, Durham, NC 27710.
FAU - Clark, M E
AU  - Clark ME
FAU - Sarngadharan, M G
AU  - Sarngadharan MG
FAU - Matthews, T J
AU  - Matthews TJ
LA  - eng
GR  - CA40660/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Gene Products, env)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Retroviridae Proteins)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
RN  - 0 (gp21 protein, Human T-lymphotropic virus 1)
RN  - 0 (gp46 protein, Human T-cell leukemia virus type I)
SB  - IM
MH  - Antibodies, Viral/immunology
MH  - Cell Line
MH  - Chromatography, Affinity
MH  - Deltaretrovirus/*analysis/immunology
MH  - *Gene Products, env
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Radioimmunoassay
MH  - Retroviridae Proteins/immunology/*isolation & purification
MH  - *Retroviridae Proteins, Oncogenic
MH  - Viral Envelope Proteins/immunology/*isolation & purification
MH  - env Gene Products, Human Immunodeficiency Virus
EDAT- 1987/12/01 00:00
MHDA- 1987/12/01 00:01
CRDT- 1987/12/01 00:00
PHST- 1987/12/01 00:00 [pubmed]
PHST- 1987/12/01 00:01 [medline]
PHST- 1987/12/01 00:00 [entrez]
AID - 0166-0934(87)90086-3 [pii]
AID - 10.1016/0166-0934(87)90086-3 [doi]
PST - ppublish
SO  - J Virol Methods. 1987 Dec;18(4):243-55. doi: 10.1016/0166-0934(87)90086-3.