PMID- 28325899
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Mar 21
TI  - Complete Genomic and Ultrastructural Analysis of a Nam Dinh Virus Isolated from 
      Culex pipiens quinquefasciatus in China.
PG  - 271
LID - 10.1038/s41598-017-00340-3 [doi]
LID - 271
AB  - The Nam Dinh virus (NDiV) was isolated from Culex quinquefasciatus in Shenzhen, 
      China, for the first time, in 2011. In this study, we characterized the 
      ultrastructure of NDiV, determined its complete genome sequence and made 
      comparisons with other known nidoviruses. Electron microscopic observation 
      revealed that the NDiV strain isolated in China produced viral nucleocapsid-like 
      particles and vesicles in host cells. The extracellular virions were enveloped 
      and were spherical with short spikes. The complete genome sequence of the newly 
      isolated NDiV was submitted to the GenBank database (GenBank accession number 
      KF522691). Sequencing of the viral genome showed that the homologies of NDiV 
      isolated in China and Vietnam were greater than 94.0% and 89.0% at the nucleotide 
      and amino acid sequence levels, respectively. Moreover, gene substitution was 
      detected, whereas insertions and deletions were not. A phylogenetic tree analysis 
      showed that these viruses belong to the genus Alphamesonivirus1 of the family 
      Mesoniviridae. The similarity between the two viruses regarding morphological and 
      molecular biological characteristics indicates that the molecular genetics of 
      NDiV are conservative and that the regional differences are unlikely to have a 
      significant effect. This is the first report of the isolation and complete 
      sequencing of a mesonivirus in mainland China.
FAU - Zhou, Jianming
AU  - Zhou J
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Jin, Yujuan
AU  - Jin Y
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Chen, Yingjian
AU  - Chen Y
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Li, Jingmei
AU  - Li J
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Zhang, Qiwen
AU  - Zhang Q
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Xie, Xianqing
AU  - Xie X
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Gan, Liping
AU  - Gan L
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China.
FAU - Liu, Qu
AU  - Liu Q
AD  - Longgang Center for Disease Control and Prevention in Shenzhen, Shenzhen, China. 
      szliuqu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170321
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - China
MH  - Cluster Analysis
MH  - Culex/*virology
MH  - *Genome, Viral
MH  - Microscopy, Electron
MH  - Nidovirales/*genetics/isolation & purification/*ultrastructure
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Virion/*ultrastructure
MH  - Whole Genome Sequencing
PMC - PMC5428213
COIS- The authors declare that they have no competing interests.
EDAT- 2017/03/23 06:00
MHDA- 2018/08/29 06:00
PMCR- 2017/03/21
CRDT- 2017/03/23 06:00
PHST- 2016/08/26 00:00 [received]
PHST- 2017/02/22 00:00 [accepted]
PHST- 2017/03/23 06:00 [entrez]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2017/03/21 00:00 [pmc-release]
AID - 10.1038/s41598-017-00340-3 [pii]
AID - 340 [pii]
AID - 10.1038/s41598-017-00340-3 [doi]
PST - epublish
SO  - Sci Rep. 2017 Mar 21;7(1):271. doi: 10.1038/s41598-017-00340-3.