PMID- 28326151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2000-2297 (Print)
IS  - 2000-2297 (Electronic)
IS  - 2000-2297 (Linking)
VI  - 9
IP  - 1
DP  - 2017
TI  - Chronic Porphyromonas gingivalis infection accelerates the occurrence of 
      age-related granules in ApoE(-)(/)(-) mice brains.
PG  - 1270602
LID - 10.1080/20002297.2016.1270602 [doi]
LID - 1270602
AB  - This study explored the origin of age-related granules in the apolipoprotein E 
      gene knockout (ApoE(-/-)) B6 background mice brains following chronic gingival 
      infection with Porphyromonas gingivalis for 24 weeks. Intracerebral localization 
      of P. gingivalis was detected by fluorescence in situ hybridization (FISH) and 
      its protease by immunohistochemistry. The age-related granules were observed by 
      periodic acid-Schiff (PAS), silver impregnation, and immunostaining. FISH showed 
      intracerebral dissemination of P. gingivalis cells (p = 0.001). PAS and silver 
      impregnation demonstrated the presence of larger inclusions restricted to the 
      CA1, CA2, and dentate gyrus sectors of the hippocampus. A specific monoclonal 
      antibody to bacterial peptidoglycan detected clusters of granules with variable 
      sizes in mice brains infected with P. gingivalis (p = 0.004), and also 
      highlighted areas of diffuse punctate staining equating to physical tissue 
      damage. Mouse immunoglobulin G was observed in the capillaries of the cerebral 
      parenchyma of all P. gingivalis-infected brains (p = 0.001), and on pyramidal 
      neurons in some severely affected mice, compared with the sham-infected mice. 
      Gingipains was also observed in microvessels of the hippocampus in the infected 
      mice. This study supports the possibility of early appearance of age-related 
      granules in ApoE(-/-) mice following inflammation-mediated tissue injury, 
      accompanied by loss of cerebral blood-brain barrier integrity.
FAU - Singhrao, Sim K
AU  - Singhrao SK
AD  - Dementia and Neurodegeneration Research Group, College of Clinical and Biomedical 
      Sciences, University of Central Lancashire , Preston , UK.
FAU - Chukkapalli, Sasanka
AU  - Chukkapalli S
AD  - Department of Periodontology, University of Florida , Gainesville , FL , USA.
FAU - Poole, Sophie
AU  - Poole S
AD  - Dementia and Neurodegeneration Research Group, College of Clinical and Biomedical 
      Sciences, University of Central Lancashire , Preston , UK.
FAU - Velsko, Irina
AU  - Velsko I
AD  - Department of Periodontology, University of Florida , Gainesville , FL , USA.
FAU - Crean, St John
AU  - Crean SJ
AD  - Dementia and Neurodegeneration Research Group, College of Clinical and Biomedical 
      Sciences, University of Central Lancashire , Preston , UK.
FAU - Kesavalu, Lakshmyya
AU  - Kesavalu L
AD  - Department of Periodontology, University of Florida, Gainesville, FL, USA; 
      Department of Oral Biology, College of Dentistry, University of Florida, 
      Gainesville, FL, USA.
LA  - eng
GR  - R01 DE020820/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20170117
PL  - United States
TA  - J Oral Microbiol
JT  - Journal of oral microbiology
JID - 101551049
PMC - PMC5328363
OTO - NOTNLM
OT  - Age-related granules
OT  - blood-brain barrier damage
OT  - infection
OT  - inflammation
OT  - injury
EDAT- 2017/03/23 06:00
MHDA- 2017/03/23 06:01
PMCR- 2017/01/01
CRDT- 2017/03/23 06:00
PHST- 2016/07/26 00:00 [received]
PHST- 2016/11/14 00:00 [revised]
PHST- 2016/11/15 00:00 [accepted]
PHST- 2017/03/23 06:00 [entrez]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2017/03/23 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 1270602 [pii]
AID - 10.1080/20002297.2016.1270602 [doi]
PST - epublish
SO  - J Oral Microbiol. 2017 Jan 17;9(1):1270602. doi: 10.1080/20002297.2016.1270602. 
      eCollection 2017.