PMID- 2832739
OWN - NLM
STAT- MEDLINE
DCOM- 19880504
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 8
IP  - 2
DP  - 1988 Feb
TI  - Temperature-sensitive transport of glycoproteins to the surface of a variant 
      mouse lymphoma cell line.
PG  - 833-42
AB  - The expression of mouse mammary tumor virus (MMTV) glycoproteins on the surface 
      of stably infected mouse lymphoma cell line W7MG1 is dramatically increased by 
      glucocorticoid hormones. A variant cell line, W7M.TS1, was selected from W7MG1 
      for its lack of expression of MMTV glycoproteins on the cell surface in response 
      to treatment with glucocorticoid. Hormonal stimulation of MMTV RNA levels and 
      hormone-induced cytolysis occurred normally in the variant cells. Furthermore, 
      the rates of production of the precursor and mature forms of MMTV glycoproteins 
      in the presence of glucocorticoid were similar in variant and wild-type cells. 
      However, the accumulation of MMTV glycoproteins on the cell surface after hormone 
      treatment was delayed by about 8 h in the variant relative to wild-type cells. 
      The steady-state level of a constitutively expressed cellular protein, T200, on 
      the variant cell surface was comparable to that on wild-type cells. However, in 
      pulse-chase experiments, the appearance of newly synthesized T200 on the cell 
      surface was delayed in the variant compared with wild-type cells. Another 
      glucocorticoid hormone response, removal of H-2 class I antigens from the cell 
      surface, was also delayed in the variant relative to wild-type cells, suggesting 
      that turnover or internalization of cell surface glycoproteins may also be 
      affected in the variant. The defects in the variant cell line were observed at 37 
      degrees C, but not at 31 degrees C; the variant cells grew normally at both 
      temperatures. This variant phenotype defines a new genetic entity that is 
      important for transport of glycoproteins between internal microsomal compartments 
      and the cell surface.
FAU - Nori, M
AU  - Nori M
AD  - Department of Pathology, University of Southern California School of Medicine, 
      Los Angeles 90033.
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
GR  - CA01149/CA/NCI NIH HHS/United States
GR  - GM36317/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - *Cell Transformation, Viral
MH  - Dexamethasone/pharmacology
MH  - Genetic Variation
MH  - Glycoproteins/genetics/*metabolism
MH  - Kinetics
MH  - Lymphoma
MH  - Mammary Tumor Virus, Mouse/drug effects/*genetics
MH  - Mice
MH  - Phenotype
MH  - Temperature
MH  - Viral Proteins/genetics/*metabolism
PMC - PMC363214
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
PMCR- 1988/02/01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
PHST- 1988/02/01 00:00 [pmc-release]
AID - 10.1128/mcb.8.2.833-842.1988 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1988 Feb;8(2):833-42. doi: 10.1128/mcb.8.2.833-842.1988.