PMID- 28327516 OWN - NLM STAT- MEDLINE DCOM- 20170622 LR - 20181113 IS - 1660-3397 (Electronic) IS - 1660-3397 (Linking) VI - 15 IP - 3 DP - 2017 Mar 22 TI - An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Abeta(25-35). LID - 10.3390/md15030083 [doi] LID - 83 AB - Increased evidence suggests that marine unsaturated fatty acids (FAs) can protect neurons from amyloid-beta (Abeta)-induced neurodegeneration. Nuclear magnetic resonance (NMR), high performance liquid chromatography (HPLC) and gas chromatography (GC) assays showed that the acetone extract 4-2A obtained from shrimp Pandalus borealis industry processing wastes contained 67.19% monounsaturated FAs and 16.84% polyunsaturated FAs. The present study evaluated the anti-oxidative and anti-inflammatory effects of 4-2A in Abeta(25-35)-insulted differentiated SH-SY5Y cells. Cell viability and cytotoxicity were measured by using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Quantitative PCR and Western blotting were used to study the expression of neurotrophins, pro-inflammatory cytokines and apoptosis-related genes. Administration of 20 muM Abeta(25-35) significantly reduced SH-SY5Y cell viability, the expression of nerve growth factor (NGF) and its tyrosine kinase TrkA receptor, as well as the level of glutathione, while increased reactive oxygen species (ROS), nitric oxide, tumor necrosis factor (TNF)-alpha, brain derived neurotrophic factor (BDNF) and its TrkB receptor. Abeta(25-35) also increased the Bax/Bcl-2 ratio and Caspase-3 expression. Treatment with 4-2A significantly attenuated the Abeta(25-35)-induced changes in cell viability, ROS, GSH, NGF, TrkA, TNF-alpha, the Bax/Bcl-2 ratio and Caspase-3, except for nitric oxide, BDNF and TrKB. In conclusion, 4-2A effectively protected SH-SY5Y cells against Abeta-induced neuronal apoptosis/death by suppressing inflammation and oxidative stress and up-regulating NGF and TrKA expression. FAU - Zhang, Yongping AU - Zhang Y AD - Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. zhangyp2015@163.com. AD - Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. zhangyp2015@163.com. FAU - Jiao, Guangling AU - Jiao G AD - Coastal Zones Research Institute Inc., 232B, avenue de l'Eglise, Shippagan, NB E8S 1J2, Canada. sebrina2006@gmail.com. AD - Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. sebrina2006@gmail.com. FAU - Song, Cai AU - Song C AD - Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. cai.song@dal.ca. AD - Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. cai.song@dal.ca. AD - Graduate Institute of Neural and Cognitive Sciences, China Medical University Hospital, Taichung 40402, Taiwan. cai.song@dal.ca. FAU - Gu, Shelly AU - Gu S AD - Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. xiaomei.gu@dal.ca. FAU - Brown, Richard E AU - Brown RE AD - Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. Richard.Brown@dal.ca. FAU - Zhang, Junzeng AU - Zhang J AD - Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Junzeng.Zhang@nrc-cnrc.gc.ca. FAU - Zhang, Pingcheng AU - Zhang P AD - Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada. pczhang2000@yahoo.com. FAU - Gagnon, Jacques AU - Gagnon J AD - Coastal Zones Research Institute Inc., 232B, avenue de l'Eglise, Shippagan, NB E8S 1J2, Canada. jacques.gagnon@umoncton.ca. FAU - Locke, Steven AU - Locke S AD - Aquatic and Crop Resource Development, National Research Council of Canada, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. Steven.Locke@nrc-cnrc.gc.ca. FAU - Stefanova, Roumiana AU - Stefanova R AD - Aquatic and Crop Resource Development, National Research Council of Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Roumiana.Stefanova@nrc-cnrc.gc.ca. FAU - Pelletier, Claude AU - Pelletier C AD - Coastal Zones Research Institute Inc., 232B, avenue de l'Eglise, Shippagan, NB E8S 1J2, Canada. Claude.pelletier@umoncton.ca. FAU - Zhang, Yi AU - Zhang Y AD - Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. hubeizhangyi@163.com. FAU - Lu, Hongyu AU - Lu H AD - Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China. irislhy@126.com. LA - eng PT - Journal Article DEP - 20170322 PL - Switzerland TA - Mar Drugs JT - Marine drugs JID - 101213729 RN - 0 (Amyloid beta-Peptides) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neuroprotective Agents) RN - 0 (Peptide Fragments) RN - 0 (Reactive Oxygen Species) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (amyloid beta-protein (25-35)) RN - 7171WSG8A2 (BDNF protein, human) RN - EC 3.4.22.- (Caspase 3) SB - IM MH - Amyloid beta-Peptides/*adverse effects/*metabolism MH - Animals MH - Apoptosis/drug effects MH - Brain-Derived Neurotrophic Factor/metabolism MH - Caspase 3/metabolism MH - Cell Line, Tumor MH - Cell Survival/drug effects MH - Crustacea/*chemistry MH - Cytoprotection/drug effects MH - Humans MH - Neuroblastoma/drug therapy MH - Neurons/*drug effects/metabolism MH - Neuroprotective Agents/*chemistry/*pharmacology MH - Neurotoxicity Syndromes/*drug therapy/metabolism MH - Oxidative Stress/drug effects MH - Peptide Fragments/*adverse effects/*metabolism MH - Reactive Oxygen Species/metabolism MH - Tumor Necrosis Factor-alpha/metabolism PMC - PMC5367040 OTO - NOTNLM OT - Acetone extract from shrimp processing by-product (4-2A) OT - Alzheimer's disease (AD) OT - Abeta25-35 OT - human neuroblastoma cell (SH-SY5Y) OT - neuroprotection OT - polyunsaturated fatty acids COIS- The authors declare no conflict of interest. EDAT- 2017/03/23 06:00 MHDA- 2017/06/24 06:00 PMCR- 2017/03/01 CRDT- 2017/03/23 06:00 PHST- 2016/12/08 00:00 [received] PHST- 2017/03/07 00:00 [revised] PHST- 2017/03/15 00:00 [accepted] PHST- 2017/03/23 06:00 [entrez] PHST- 2017/03/23 06:00 [pubmed] PHST- 2017/06/24 06:00 [medline] PHST- 2017/03/01 00:00 [pmc-release] AID - md15030083 [pii] AID - marinedrugs-15-00083 [pii] AID - 10.3390/md15030083 [doi] PST - epublish SO - Mar Drugs. 2017 Mar 22;15(3):83. doi: 10.3390/md15030083.