PMID- 28327951
OWN - NLM
STAT- MEDLINE
DCOM- 20180302
LR  - 20220330
IS  - 1569-8041 (Electronic)
IS  - 0923-7534 (Print)
IS  - 0923-7534 (Linking)
VI  - 28
IP  - 5
DP  - 2017 May 1
TI  - HPV16 DNA and integration in normal and malignant epithelium: implications for 
      the etiology of laryngeal squamous cell carcinoma.
PG  - 1105-1110
LID - 10.1093/annonc/mdx027 [doi]
AB  - BACKGROUND: Molecular evidence suggests that human papillomavirus (HPV) has a 
      role in the etiology of oropharyngeal squamous cell carcinoma. However, the role 
      of HPV in laryngeal squamous cell carcinoma (LSCC) is unclear. PATIENTS AND 
      METHODS: We conducted a case-control study using tumor tissue specimens from 300 
      LSCC patients and vocal cord polyp specimens from 300 cancer-free controls. HPV 
      genotype, HPV16 viral load and viral integration status, and p16 expression were 
      determined. RESULTS: The prevalence of HPV (all types) was higher in cases than 
      controls [21 (7.0%) versus 10 (3.3%), adjusted odds ratio (aOR) 2.37, 95% CI 
      1.08-5.21]. The prevalence of HPV16 was higher in cases than controls [20 (6.7%) 
      versus 8 (2.7%), aOR 2.84, 95% CI 1.21-6.68]. The risk of LSCC associated with 
      HPV16 DNA positivity was even higher in patients aged 55 years or younger (aOR 
      3.52, 95% CI 1.07-11.54), males (aOR 4.74, 95% CI 1.33-16.90), never-smokers (aOR 
      5.57, 95% CI 1.41-22.10), and never-drinkers (aOR 3.72, 95% CI 1.09-12.72). HPV 
      DNA was partly or fully integrated in all 20 HPV16-positive cases but was 
      episomal in all 8 HPV16-positive controls; however, the HPV16-positive cases and 
      controls had similar viral loads (P = 0.28). P16 immunostaining was positive in 
      31 of the 300 cases (10.3%) and negative in all 300 controls. CONCLUSION: These 
      results suggest that prior infection with HPV16 may play a role in the etiology 
      of some LSCC. This larger case-control study will offer for the first time the 
      possibility to address in depth the understanding of a tissue-specific role of 
      HPV in laryngeal carcinogenesis. Further studies with larger samples are needed 
      to confirm these findings.
CI  - (c) The Author 2017. Published by Oxford University Press on behalf of the European 
      Society for Medical Oncology.
FAU - Chen, X
AU  - Chen X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Gao, L
AU  - Gao L
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Sturgis, E M
AU  - Sturgis EM
AD  - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer 
      Center, Houston, USA.
FAU - Liang, Z
AU  - Liang Z
AD  - Department of Pathology, Peking Union Medical College Hospital, Peking Union 
      Medical College and Chinese Academy of Medical Sciences, Beijing, China.
FAU - Zhu, Y
AU  - Zhu Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Xia, X
AU  - Xia X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Zhu, X
AU  - Zhu X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Chen, X
AU  - Chen X
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
FAU - Li, G
AU  - Li G
AD  - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer 
      Center, Houston, USA.
FAU - Gao, Z
AU  - Gao Z
AD  - Department of Otolaryngology-Head and Neck Surgery, Peking Union Medical College 
      Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 
      Beijing, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Oncol
JT  - Annals of oncology : official journal of the European Society for Medical 
      Oncology
JID - 9007735
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/pathology/*virology
MH  - DNA, Viral/*genetics/isolation & purification
MH  - Epithelium/pathology/virology
MH  - Female
MH  - Human papillomavirus 16/*genetics/pathogenicity
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/pathology/*virology
MH  - Male
MH  - Middle Aged
MH  - Viral Load
MH  - Virus Integration/genetics
PMC - PMC5406756
OTO - NOTNLM
OT  - head and neck cancer
OT  - human papillomavirus
OT  - laryngeal cancer
OT  - physical status
OT  - risk
OT  - viral load
EDAT- 2017/03/23 06:00
MHDA- 2018/03/03 06:00
PMCR- 2017/01/23
CRDT- 2017/03/23 06:00
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2018/03/03 06:00 [medline]
PHST- 2017/03/23 06:00 [entrez]
PHST- 2017/01/23 00:00 [pmc-release]
AID - S0923-7534(19)32006-X [pii]
AID - mdx027 [pii]
AID - 10.1093/annonc/mdx027 [doi]
PST - ppublish
SO  - Ann Oncol. 2017 May 1;28(5):1105-1110. doi: 10.1093/annonc/mdx027.