PMID- 28329151
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20171226
IS  - 1758-535X (Electronic)
IS  - 1079-5006 (Linking)
VI  - 73
IP  - 1
DP  - 2017 Dec 12
TI  - Caloric Restriction and Rapamycin Differentially Alter Energy Metabolism in 
      Yeast.
PG  - 29-38
LID - 10.1093/gerona/glx024 [doi]
AB  - Rapamycin (RM), a drug that inhibits the mechanistic target of rapamycin (mTOR) 
      pathway and responds to nutrient availability, seemingly mimics the effects of 
      caloric restriction (CR) on healthy life span. However, the extent of the 
      mechanistic overlap between RM and CR remains incompletely understood. Here, we 
      compared the impact of CR and RM on cellular metabolic status. Both regimens 
      maintained intracellular ATP through the chronological aging process and showed 
      enhanced mitochondrial capacity. Comparative transcriptome analysis showed that 
      CR had a stronger impact on global gene expression than RM. We observed a like 
      impact on the metabolome and identified distinct metabolites affected by CR and 
      RM. CR severely reduced the level of energy storage molecules including glycogen 
      and lipid droplets, whereas RM did not. RM boosted the production of enzymes 
      responsible for the breakdown of glycogen and lipid droplets. Collectively, these 
      results provide insights into the distinct energy metabolism mechanisms induced 
      by CR and RM, suggesting that these two anti-aging regimens might extend life 
      span through distinctive pathways.
CI  - (c) The Author(s) 2017. Published by Oxford University Press on behalf of The 
      Gerontological Society of America. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Choi, Kyung-Mi
AU  - Choi KM
AD  - Institute of Animal Molecular Biotechnology, Korea University, Seoul, Republic of 
      Korea.
FAU - Hong, Seok-Jin
AU  - Hong SJ
AD  - Division of Biotechnology, College of Life Sciences & Biotechnology, Korea 
      University, Seoul, Republic of Korea.
FAU - van Deursen, Jan M
AU  - van Deursen JM
AD  - Departments of Biochemistry and Molecular Biology and Pediatric and Adolescent 
      Medicine, Mayo Clinic, Rochester, Minnesota.
FAU - Kim, Sooah
AU  - Kim S
AD  - Department of Biotechnology, Graduate School, Korea University, Seoul, Republic 
      of Korea.
FAU - Kim, Kyoung Heon
AU  - Kim KH
AD  - Department of Biotechnology, Graduate School, Korea University, Seoul, Republic 
      of Korea.
FAU - Lee, Cheol-Koo
AU  - Lee CK
AD  - Institute of Animal Molecular Biotechnology, Korea University, Seoul, Republic of 
      Korea.
AD  - Division of Biotechnology, College of Life Sciences & Biotechnology, Korea 
      University, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Gerontol A Biol Sci Med Sci
JT  - The journals of gerontology. Series A, Biological sciences and medical sciences
JID - 9502837
RN  - 0 (RNA, Fungal)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - *Aging
MH  - *Caloric Restriction
MH  - Flow Cytometry
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Immunoblotting
MH  - Metabolome/genetics
MH  - Microarray Analysis
MH  - Oxygen Consumption
MH  - RNA, Fungal/*analysis
MH  - Saccharomyces cerevisiae/cytology/genetics/*metabolism
MH  - Sirolimus/*pharmacology
OTO - NOTNLM
OT  - Metabolomics
OT  - Transcriptomics
EDAT- 2017/03/23 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/03/23 06:00
PHST- 2016/07/26 00:00 [received]
PHST- 2017/02/01 00:00 [accepted]
PHST- 2017/03/23 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
PHST- 2017/03/23 06:00 [entrez]
AID - 3063959 [pii]
AID - 10.1093/gerona/glx024 [doi]
PST - ppublish
SO  - J Gerontol A Biol Sci Med Sci. 2017 Dec 12;73(1):29-38. doi: 
      10.1093/gerona/glx024.