PMID- 28330136
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 6
IP  - 1
DP  - 2016 Jun
TI  - Enhanced production of thermostable amidase from Geobacillus subterraneus RL-2a 
      MTCC 11502 via optimization of physicochemical parameters using Taguchi DOE 
      methodology.
PG  - 66
LID - 10.1007/s13205-016-0390-1 [doi]
LID - 66
AB  - The specific effect of chemical and physical factors on amidase production from 
      Geobacillus subterraneus RL-2a was investigated using design of experiments (DOE) 
      methodology. The one-factor-at-a-time (OFAT) method was used to study the effects 
      of carbon and nitrogen sources on amidase production. Subsequently, optimal 
      levels of physical parameters and key media components, namely temperature, pH, 
      sucrose, K(2)HPO(4), NaCl, yeast, CaCl(2).2H(2)O and MgSO(4).7H(2)O, were 
      determined using the Taguchi orthogonal array (OA) experimental design (DOE) 
      methodology. Taguchi method based on three levels with a OA layout of L18 
      (2(1) x 3(7)) with eight most influential factors on amidase synthesis for the 
      proposed experimental design. Analysis of variance was performed on the obtained 
      results and optimum condition suggested by statistical calculations was tested in 
      a verification test. An increase of 169.56 % in amidase production compared to 
      the unoptimized conditions was observed and the conversion of isonicotinamide was 
      significantly improved after performing optimization techniques, including OFAT 
      and Taguchi method. The result indicated that Taguchi method was effective in 
      optimizing the culture conditions of amidase production.
FAU - Mehta, Praveen Kumar
AU  - Mehta PK
AD  - Department of Biotechnology, Himachal Pradesh University, Simla, 171 005, India.
FAU - Bhatia, Shashi Kant
AU  - Bhatia SK
AD  - Department of Biotechnology, Himachal Pradesh University, Simla, 171 005, India.
FAU - Bhatia, Ravi Kant
AU  - Bhatia RK
AD  - Department of Biotechnology, Himachal Pradesh University, Simla, 171 005, India.
FAU - Bhalla, Tek Chand
AU  - Bhalla TC
AD  - Department of Biotechnology, Himachal Pradesh University, Simla, 171 005, India. 
      bhallatc@rediffmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160215
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC4754296
OTO - NOTNLM
OT  - Amidase
OT  - Geobacillus subterraneus
OT  - Optimization
OT  - Orthogonal array
OT  - Production
COIS- The authors declare that they have no conflict of interest in the publication.
EDAT- 2016/01/01 00:00
MHDA- 2016/01/01 00:01
PMCR- 2016/02/15
CRDT- 2017/03/24 06:00
PHST- 2015/09/04 00:00 [received]
PHST- 2015/10/22 00:00 [accepted]
PHST- 2017/03/24 06:00 [entrez]
PHST- 2016/01/01 00:00 [pubmed]
PHST- 2016/01/01 00:01 [medline]
PHST- 2016/02/15 00:00 [pmc-release]
AID - 10.1007/s13205-016-0390-1 [pii]
AID - 390 [pii]
AID - 10.1007/s13205-016-0390-1 [doi]
PST - ppublish
SO  - 3 Biotech. 2016 Jun;6(1):66. doi: 10.1007/s13205-016-0390-1. Epub 2016 Feb 15.