PMID- 28336908
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20190609
IS  - 2329-0358 (Electronic)
IS  - 1425-9524 (Linking)
VI  - 22
DP  - 2017 Mar 24
TI  - Early Chimerism After Liver Transplantation Reflects the Clinical Course of 
      Recurrent Hepatitis C.
PG  - 156-165
AB  - BACKGROUND Human leukocyte antigen (HLA) mismatch is a characteristic feature of 
      post-orthotopic liver transplantation (OLT) hepatitis C. To investigate the 
      importance of donor HLA-restricted immune cells in post-OLT hepatitis C 
      recurrence, we analyzed the frequency of donor chimerism and the clinical course 
      of post-OLT hepatitis C. MATERIAL AND METHODS We analyzed peripheral blood 
      chimerism in 11 HCV-reinfected patients with post-HLA mismatched OLT. Patients 
      were divided into 2 groups: the OLT chronic hepatitis C (CHC) group (n=8), 
      exhibiting active hepatitis C recurrence; and the OLT-persistently normal ALT 
      (PNALT) group (n=3), without active hepatitis. Chimerism was analyzed by flow 
      cytometry using donor-specific anti-HLA antibodies in peripheral blood 
      mononuclear cells from 1-100 days after OLT. Kidney (n=7) and lung (n=7) 
      transplant recipients were also analyzed for comparison. As immune cells from the 
      donor liver might contribute to post-OLT chimerism, the characteristics of 
      perfusates from donor livers (n=10) were analyzed and defined. RESULTS 
      Donor-derived cells were frequently observed in liver and lung transplant 
      recipients. The frequency of donor-derived cells from the B cell subset was 
      significantly higher in peripheral blood from OLT-CHC group than in that of the 
      OLT-PNALT group. B cells, however, were not the predominant subset in the 
      perfusates, indicating that inflow of donor-derived cells alone did not cause the 
      chimerism. CONCLUSIONS Chimerism of B cells is frequent in liver transplant 
      patients with early recurrence of hepatitis C. We propose that monitoring of 
      early chimerism could facilitate early detection of chronic hepatitis C 
      recurrence, although we need more cases to investigate.
FAU - Utsumi, Masashi
AU  - Utsumi M
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Takaki, Akinobu
AU  - Takaki A
AD  - Department of Gastroenterology and Hepatology, Okayama University Graduate School 
      of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Umeda, Yuzo
AU  - Umeda Y
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Koike, Kazuko
AU  - Koike K
AD  - Department of Gastroenterology and Hepatology, Okayama University Graduate School 
      of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Napier, Stephanie C
AU  - Napier SC
AD  - Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical 
      Science, The University of Tokyo, Tokyo, Japan.
FAU - Watanabe, Nobukazu
AU  - Watanabe N
AD  - Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical 
      Science, The University of Tokyo, Tokyo, Japan.
FAU - Shinoura, Susumu
AU  - Shinoura S
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Yoshida, Ryuichi
AU  - Yoshida R
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Nobuoka, Daisuke
AU  - Nobuoka D
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Yasunaka, Tetsuya
AU  - Yasunaka T
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Oto, Takahiro
AU  - Oto T
AD  - Department of Thoracic Surgery, Okayama University Graduate School of Medicine, 
      Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Araki, Motoo
AU  - Araki M
AD  - Department of Urology, Okayama University Graduate School of Medicine, Dentistry, 
      and Pharmaceutical Sciences, Okayama, Japan.
FAU - Yamamoto, Kazuhide
AU  - Yamamoto K
AD  - Department of Gastroenterology and Hepatology, Okayama University Graduate School 
      of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Fujiwara, Toshiyoshi
AU  - Fujiwara T
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
FAU - Yagi, Takahito
AU  - Yagi T
AD  - Department of Gastroenterological Surgery, Okayama University Graduate School of 
      Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170324
PL  - United States
TA  - Ann Transplant
JT  - Annals of transplantation
JID - 9802544
RN  - 0 (Autoantibodies)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Autoantibodies
MH  - B-Lymphocytes/immunology
MH  - *Chimerism
MH  - Female
MH  - Flow Cytometry
MH  - HLA Antigens/*immunology
MH  - Hepatitis C/*immunology
MH  - Humans
MH  - Liver Failure/immunology/*surgery
MH  - Liver Transplantation/*adverse effects
MH  - Male
MH  - Middle Aged
MH  - Recurrence
EDAT- 2017/03/25 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/03/25 06:00
PHST- 2017/03/25 06:00 [entrez]
PHST- 2017/03/25 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
AID - 900494 [pii]
AID - 10.12659/aot.900494 [doi]
PST - epublish
SO  - Ann Transplant. 2017 Mar 24;22:156-165. doi: 10.12659/aot.900494.