PMID- 28339033
OWN - NLM
STAT- MEDLINE
DCOM- 20180122
LR  - 20180122
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 15
IP  - 5
DP  - 2017 May
TI  - Role of tropomyosin in silkworm allergy.
PG  - 3264-3270
LID - 10.3892/mmr.2017.6373 [doi]
AB  - Silkworm pupae are widely consumed in Asian countries and allergic reactions 
      following consumption have been described. However, false‑positive responses in 
      skin prick allergy tests or non‑specific immunoglobulin E (IgE) responses to 
      total extract of silkworm pupa make diagnosis difficult. Although improved 
      allergy diagnosis is required, molecular characterization of silkworm allergens 
      has not been performed to date, except for Bomb m 1, an arginine kinase. This 
      study aimed to evaluate the allergenicity of tropomyosin, a well‑established 
      invertebrate pan‑allergen, from silkworm pupa. The silkworm tropomyosin gene was 
      cloned by reverse transcription and polymerase chain reaction, and the protein 
      was overexpressed in Escherichia coli and purified by affinity chromatography 
      using Nickel‑resin. IgE reactivity of the recombinant protein was examined by 
      ELISA and competitive inhibition analyses. Silkworm pupa tropomyosin shared 
      73.5‑92.3% amino acid sequence identity with previously identified allergenic 
      tropomyosins. Sera from eight of 15 patients with silkworm allergy (53.3%) 
      exhibited binding of IgE to the recombinant protein. However, recombinant protein 
      was able to inhibit less than 10% of IgE reactivity to silkworm pupa extract. Of 
      the eight sera tested, six that specifically reacted with silkworm tropomyosin 
      also demonstrated IgE reactivity to shrimp and crab. In the present study, 
      specific IgE to silkworm tropomyosin was detected in patients with silkworm 
      allergy, suggesting that it may be useful in diagnosis of allergy to silkworm 
      pupa.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
FAU - Han, In-Soo
AU  - Han IS
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
FAU - Lee, June Yong
AU  - Lee JY
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
FAU - Park, Kyung Hee
AU  - Park KH
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
FAU - Lee, Jae-Hyun
AU  - Lee JH
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
FAU - Park, Jung-Won
AU  - Park JW
AD  - Department of Internal Medicine, Institute of Allergy, Yonsei University College 
      of Medicine, Seoul 03722, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20170324
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Allergens)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tropomyosin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/*immunology/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen-Antibody Reactions
MH  - Bombyx/growth & development/metabolism
MH  - Brachyura/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Hypersensitivity/immunology/*pathology
MH  - Immunoglobulin E/blood/immunology
MH  - Male
MH  - Pupa/metabolism
MH  - Recombinant Proteins/biosynthesis/immunology/isolation & purification
MH  - Sequence Alignment
MH  - Skin Tests
MH  - Tropomyosin/genetics/*immunology/metabolism
MH  - Young Adult
EDAT- 2017/03/25 06:00
MHDA- 2018/01/23 06:00
CRDT- 2017/03/25 06:00
PHST- 2016/01/27 00:00 [received]
PHST- 2017/01/27 00:00 [accepted]
PHST- 2017/03/25 06:00 [pubmed]
PHST- 2018/01/23 06:00 [medline]
PHST- 2017/03/25 06:00 [entrez]
AID - 10.3892/mmr.2017.6373 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 May;15(5):3264-3270. doi: 10.3892/mmr.2017.6373. Epub 2017 Mar 
      24.