PMID- 28340426 OWN - NLM STAT- MEDLINE DCOM- 20170915 LR - 20180314 IS - 1872-9142 (Electronic) IS - 0161-5890 (Linking) VI - 85 DP - 2017 May TI - Porcine parvovirus induces activation of NF-kappaB signaling pathways in PK-15 cells mediated by toll-like receptors. PG - 248-255 LID - S0161-5890(16)30262-0 [pii] LID - 10.1016/j.molimm.2016.12.002 [doi] AB - Porcine parvovirus (PPV) is a pathogenic factor that primarily induces severe reproductive failure of pregnant swine, which results in extensive losses to the swine industry worldwide. In this study, a potential mechanism of PPV-induced activation of the nuclear transcription factor-kappaB (NF-kappaB) by infection in porcine kidney cells (PK-15) was elucidated for the first time. The subcellular localization of p65 analyzed by immunofluorescence assay (IFA) showed that PPV infection induced p65 translocation from the cytoplasm to the nucleus. p65 phosphorylation was detected in PK-15 cells with progression of PPV infection. NF-kappaB-regulated gene expression was enhanced in a viral dose-dependent manner using the NF-kappaB luciferase reporter assay system. Furthermore, PPV-induced NF-kappaB activation was closely related to the inhibitory kappa B alpha (IkappaBalpha) degradation. Treatment with a NF-kappaB-specific inhibitor demonstrated that the production of PPV progeny viruses was enhanced to some extent. In addition, these results demonstrated that the adapter molecule TIR domain-containing adapter inducing IFN-beta (TRIF) and myeloid differentiation primary-response protein 88 (MyD88)-dependent signaling pathways were involved in PPV-induced NF-kappaB activation. Together, these results provide evidence that the toll-like receptor (TLR) pathway participates in recognition of PPV and induction of NF-kappaB activation, and add to understanding of the molecular mechanisms underlying PPV infection. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Cao, Liyan AU - Cao L AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Chen, Jianfei AU - Chen J AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Wei, Yanwu AU - Wei Y AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Shi, Hongyan AU - Shi H AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Zhang, Xin AU - Zhang X AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Yuan, Jing AU - Yuan J AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Shi, Da AU - Shi D AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Liu, Jianbo AU - Liu J AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Zhu, Xiangdong AU - Zhu X AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Wang, Xin AU - Wang X AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Cui, Shangjin AU - Cui S AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. FAU - Feng, Li AU - Feng L AD - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China. Electronic address: fl@hvri.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170321 PL - England TA - Mol Immunol JT - Molecular immunology JID - 7905289 RN - 0 (NF-kappa B) RN - 0 (Toll-Like Receptors) SB - IM MH - Animals MH - Blotting, Western MH - Cell Line MH - Fluorescent Antibody Technique MH - NF-kappa B/*immunology/metabolism MH - Parvoviridae Infections/*veterinary MH - Parvovirus, Porcine/*immunology MH - Real-Time Polymerase Chain Reaction MH - Signal Transduction/*immunology MH - Swine MH - Toll-Like Receptors/*immunology/metabolism EDAT- 2017/03/25 06:00 MHDA- 2017/09/16 06:00 CRDT- 2017/03/25 06:00 PHST- 2016/08/15 00:00 [received] PHST- 2016/11/30 00:00 [revised] PHST- 2016/12/01 00:00 [accepted] PHST- 2017/03/25 06:00 [pubmed] PHST- 2017/09/16 06:00 [medline] PHST- 2017/03/25 06:00 [entrez] AID - S0161-5890(16)30262-0 [pii] AID - 10.1016/j.molimm.2016.12.002 [doi] PST - ppublish SO - Mol Immunol. 2017 May;85:248-255. doi: 10.1016/j.molimm.2016.12.002. Epub 2017 Mar 21.