PMID- 28340575 OWN - NLM STAT- MEDLINE DCOM- 20180102 LR - 20230801 IS - 1742-2094 (Electronic) IS - 1742-2094 (Linking) VI - 14 IP - 1 DP - 2017 Mar 24 TI - NOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury. PG - 65 LID - 10.1186/s12974-017-0843-4 [doi] LID - 65 AB - BACKGROUND: NADPH oxidase (NOX2) is an enzyme system that generates reactive oxygen species (ROS) in microglia and macrophages. Excessive ROS production is linked with neuroinflammation and chronic neurodegeneration following traumatic brain injury (TBI). Redox signaling regulates macrophage/microglial phenotypic responses (pro-inflammatory versus anti-inflammatory), and NOX2 inhibition following moderate-to-severe TBI markedly reduces pro-inflammatory activation of macrophages/microglia resulting in concomitant increases in anti-inflammatory responses. Here, we report the signaling pathways that regulate NOX2-dependent macrophage/microglial phenotype switching in the TBI brain. METHODS: Bone marrow-derived macrophages (BMDMs) prepared from wildtype (C57Bl/6) and NOX2 deficient (NOX2(-/-)) mice were treated with lipopolysaccharide (LPS; 10 ng/ml), interleukin-4 (IL-4; 10 ng/ml), or combined LPS/IL-4 to investigate signal transduction pathways associated with macrophage activation using western immunoblotting and qPCR analyses. Signaling pathways and activation markers were evaluated in ipsilateral cortical tissue obtained from adult male wildtype and NOX2(-/-) mice that received moderate-level controlled cortical impact (CCI). A neutralizing anti-IL-10 approach was used to determine the effects of IL-10 on NOX2-dependent transitions from pro- to anti-inflammatory activation states. RESULTS: Using an LPS/IL-4-stimulated BMDM model that mimics the mixed pro- and anti-inflammatory responses observed in the injured cortex, we show that NOX2(-/-) significantly reduces STAT1 signaling and markers of pro-inflammatory activation. In addition, NOX2(-/-) BMDMs significantly increase anti-inflammatory marker expression; IL-10-mediated STAT3 signaling, but not STAT6 signaling, appears to be critical in regulating this anti-inflammatory response. Following moderate-level CCI, IL-10 is significantly increased in microglia/macrophages in the injured cortex of NOX2(-/-) mice. These changes are associated with increased STAT3 activation, but not STAT6 activation, and a robust anti-inflammatory response. Neutralization of IL-10 in NOX2(-/-) BMDMs or CCI mice blocks STAT3 activation and the anti-inflammatory response, thereby demonstrating a critical role for IL-10 in regulating NOX2-dependent transitions between pro- and anti-inflammatory activation states. CONCLUSIONS: These studies indicate that following TBI NOX2 inhibition promotes a robust anti-inflammatory response in macrophages/microglia that is mediated by the IL-10/STAT3 signaling pathway. Thus, therapeutic interventions that inhibit macrophage/microglial NOX2 activity may improve TBI outcomes by not only limiting pro-inflammatory neurotoxic responses, but also enhancing IL-10-mediated anti-inflammatory responses that are neuroprotective. FAU - Barrett, James P AU - Barrett JP AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. FAU - Henry, Rebecca J AU - Henry RJ AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. FAU - Villapol, Sonia AU - Villapol S AD - Laboratory for Brain Injury and Dementia, Department of Neuroscience, Georgetown University, Washington, DC, USA. FAU - Stoica, Bogdan A AU - Stoica BA AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. FAU - Kumar, Alok AU - Kumar A AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. FAU - Burns, Mark P AU - Burns MP AD - Laboratory for Brain Injury and Dementia, Department of Neuroscience, Georgetown University, Washington, DC, USA. FAU - Faden, Alan I AU - Faden AI AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. FAU - Loane, David J AU - Loane DJ AD - Department of Anesthesiology and Shock, Trauma and Anesthesiology Research (STAR) Center, University of Maryland School of Medicine, 655 West Baltimore Street, #6-011, Baltimore, MD, 21201, USA. dloane@anes.umm.edu. LA - eng GR - R03 NS067417/NS/NINDS NIH HHS/United States GR - P30 AG028747/AG/NIA NIH HHS/United States GR - R01 NS037313/NS/NINDS NIH HHS/United States GR - R01 NS082308/NS/NINDS NIH HHS/United States GR - R01 NS046717/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20170324 PL - England TA - J Neuroinflammation JT - Journal of neuroinflammation JID - 101222974 RN - 0 (IL10 protein, mouse) RN - 0 (STAT3 Transcription Factor) RN - 0 (Stat3 protein, mouse) RN - 130068-27-8 (Interleukin-10) RN - EC 1.6.3.- (Cybb protein, mouse) RN - EC 1.6.3.- (NADPH Oxidase 2) SB - IM MH - Animals MH - Brain Injuries, Traumatic/*immunology/metabolism/pathology MH - Inflammation/immunology/metabolism/*pathology MH - Interleukin-10/immunology/metabolism MH - Macrophage Activation/*immunology MH - Macrophages/*immunology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - NADPH Oxidase 2/*deficiency MH - Phenotype MH - STAT3 Transcription Factor/immunology/metabolism PMC - PMC5366128 OTO - NOTNLM OT - Interleukin-10 OT - Macrophage OT - NADPH oxidase OT - NOX2 OT - Neuroinflammation OT - Traumatic brain injury EDAT- 2017/03/28 06:00 MHDA- 2018/01/03 06:00 PMCR- 2017/03/24 CRDT- 2017/03/26 06:00 PHST- 2017/01/25 00:00 [received] PHST- 2017/03/16 00:00 [accepted] PHST- 2017/03/26 06:00 [entrez] PHST- 2017/03/28 06:00 [pubmed] PHST- 2018/01/03 06:00 [medline] PHST- 2017/03/24 00:00 [pmc-release] AID - 10.1186/s12974-017-0843-4 [pii] AID - 843 [pii] AID - 10.1186/s12974-017-0843-4 [doi] PST - epublish SO - J Neuroinflammation. 2017 Mar 24;14(1):65. doi: 10.1186/s12974-017-0843-4.