PMID- 28341632
OWN - NLM
STAT- MEDLINE
DCOM- 20170821
LR  - 20200930
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Linking)
VI  - 312
IP  - 5
DP  - 2017 May 1
TI  - Microvascular leakage in acute myocardial infarction: characterization by 
      histology, biochemistry, and magnetic resonance imaging.
PG  - H1068-H1075
LID - 10.1152/ajpheart.00073.2017 [doi]
AB  - Cardiac microvascular obstruction (MVO) after ischemia-reperfusion (I/R) has been 
      well studied, but microvascular leakage (MVL) remains largely unexplored. We 
      characterized MVL in the mouse I/R model by histology, biochemistry, and cardiac 
      magnetic resonance (CMR) imaging. I/R was induced surgically in mice. MVL was 
      determined by administrating the microvascular permeability tracer Evans blue 
      (EB) and/or gadolinium-diethylenetriaminepentaacetic acid contrast. The size of 
      MVL, infarction, and MVO in the heart was quantified histologically. Myocardial 
      EB was extracted and quantified chromatographically. Serial CMR images were 
      acquired from euthanized mice to determine late gadolinium enhancement (LGE) for 
      comparison with MVL quantified by histology. I/R resulted in MVL with its 
      severity dependent on the ischemic duration and reaching its maximum at 24-48 h 
      after reperfusion. The size of MVL correlated with the degree of left ventricular 
      dilatation and reduction in ejection fraction. Within the risk zone, the area of 
      MVL (75 +/- 2%) was greater than that of infarct (47 +/- 4%, P < 0.01) or MVO 
      (36 +/- 4%, P < 0.01). Contour analysis of paired CMR-LGE by CMR and histological 
      MVL images revealed a high degree of spatial colocalization (r = 0.959, P < 
      0.0001). These data indicate that microvascular barrier function is damaged after 
      I/R leading to MVL. Histological and biochemical means are able to characterize 
      MVL by size and severity while CMR-LGE is a potential diagnostic tool for MVL. 
      The size of ischemic myocardium exhibiting MVL was greater than that of 
      infarction and MVO, implying a role of MVL in postinfarct pathophysiology.NEW & 
      NOTEWORTHY We characterized, for the first time, the features of microvascular 
      leakage (MVL) as a consequence of reperfused myocardial infarction. The size of 
      ischemic myocardium exhibiting MVL was significantly greater than that of 
      infarction or no reflow. We made a proof-of-concept finding on the diagnostic 
      potential of MVL by cardiac magnetic resonance imaging.
CI  - Copyright (c) 2017 the American Physiological Society.
FAU - Gao, Xiao-Ming
AU  - Gao XM
AD  - Experimental Cardiology Laboratory, Baker Heart and Diabetes Institute, 
      Melbourne, Victoria, Australia.
AD  - Department of Surgery/Medicine, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
FAU - Wu, Qi-Zhu
AU  - Wu QZ
AD  - Monash Biomedical Imaging, Monash University, Melbourne, Victoria, Australia; 
      and.
FAU - Kiriazis, Helen
AU  - Kiriazis H
AD  - Experimental Cardiology Laboratory, Baker Heart and Diabetes Institute, 
      Melbourne, Victoria, Australia.
FAU - Su, Yidan
AU  - Su Y
AD  - Experimental Cardiology Laboratory, Baker Heart and Diabetes Institute, 
      Melbourne, Victoria, Australia.
FAU - Han, Li-Ping
AU  - Han LP
AD  - Experimental Cardiology Laboratory, Baker Heart and Diabetes Institute, 
      Melbourne, Victoria, Australia.
FAU - Pearson, James Todd
AU  - Pearson JT
AD  - Monash Biomedical Imaging, Monash University, Melbourne, Victoria, Australia; 
      and.
FAU - Taylor, Andrew J
AU  - Taylor AJ
AD  - Department of Cardiovascular Medicine, Alfred Hospital, Melbourne, Victoria, 
      Australia.
FAU - Du, Xiao-Jun
AU  - Du XJ
AD  - Experimental Cardiology Laboratory, Baker Heart and Diabetes Institute, 
      Melbourne, Victoria, Australia; xiao-jun.du@baker.edu.au.
AD  - Department of Surgery/Medicine, Central Clinical School, Monash University, 
      Melbourne, Victoria, Australia.
LA  - eng
PT  - Journal Article
DEP - 20170324
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
SB  - IM
MH  - Animals
MH  - Capillary Permeability
MH  - Hemorrhage/*diagnostic imaging/etiology/*pathology
MH  - Magnetic Resonance Imaging, Cine/*methods
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Microvessels/diagnostic imaging/*pathology
MH  - Myocardial Infarction/complications/*diagnostic imaging/*pathology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - cardiac magnetic resonance
OT  - infarct size
OT  - microvascular damage
OT  - microvascular leakage
OT  - myocardial infarction
EDAT- 2017/03/28 06:00
MHDA- 2017/08/22 06:00
CRDT- 2017/03/26 06:00
PHST- 2017/02/03 00:00 [received]
PHST- 2017/03/09 00:00 [revised]
PHST- 2017/03/13 00:00 [accepted]
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2017/08/22 06:00 [medline]
PHST- 2017/03/26 06:00 [entrez]
AID - ajpheart.00073.2017 [pii]
AID - 10.1152/ajpheart.00073.2017 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2017 May 1;312(5):H1068-H1075. doi: 
      10.1152/ajpheart.00073.2017. Epub 2017 Mar 24.