PMID- 28342550
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180326
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 63
DP  - 2017 Jun
TI  - The effects of endothelin-1 on satellite glial cells in peripheral ganglia.
PG  - 37-42
LID - S0143-4179(16)30176-7 [pii]
LID - 10.1016/j.npep.2017.03.002 [doi]
AB  - Endothelins (ET) are a family of highly active neuropeptides with manifold 
      influences via ET receptors (ETR) in both the peripheral and central nervous 
      systems. We have shown previously that satellite glial cells (SGCs) in mouse 
      trigeminal ganglia (TG) are extremely sensitive to ET-1 in evoking [Ca(2+)](in) 
      increase, apparently via ET(B)R activation, but there is no functional 
      information on ETR in SGCs of other peripheral ganglia. Here we tested the 
      effects of ET-1 on SGCs in nodose ganglia (NG), which is sensory, and superior 
      cervical ganglia (Sup-CG), which is part of the sympathetic nervous system, and 
      further investigated the influence of ET-1 on SGCs in TG. Using calcium imaging 
      we found that SGCs in intact, freshly isolated NG and Sup-CG are highly sensitive 
      to ET-1, with threshold concentration at 0.1nM. Our results showed that 
      [Ca(2+)](in) elevation in response to ET-1 was partially due to Ca(2+) influx 
      from the extracellular space and partially to Ca(2+) release from intracellular 
      stores. Using receptor selective ETR agonists and antagonists, we found that the 
      responses were mediated by mixed ET(A)R/ET(B)R in SGCs of NG and predominantly by 
      ET(B)R in SGCs of Sup-CG. By employing intracellular dye injection we examined 
      coupling among SGCs around different neurons in the presence of 5nM ET-1 and 
      observed coupling inhibition in all the three ganglion types. In summary, our 
      work showed that SGCs in mouse sensory and sympathetic ganglia are highly 
      sensitive to ET-1 and that this peptide markedly reduces SGCs coupling. We 
      conclude that ET-1, which may participate in neuron-glia communications, has 
      similar functions in wide range of peripheral ganglia.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Feldman-Goriachnik, Rachel
AU  - Feldman-Goriachnik R
AD  - Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, 
      Mount Scopus, Jerusalem 91240, Israel.
FAU - Hanani, Menachem
AU  - Hanani M
AD  - Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, 
      Mount Scopus, Jerusalem 91240, Israel. Electronic address: hananim@cc.huji.ac.il.
LA  - eng
PT  - Journal Article
DEP - 20170318
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Endothelin Receptor Antagonists)
RN  - 0 (Endothelin-1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Endothelin Receptor Antagonists/pharmacology
MH  - Endothelin-1/*pharmacology
MH  - Ganglia, Sympathetic/*drug effects/metabolism
MH  - Mice
MH  - Satellite Cells, Perineuronal/*drug effects/metabolism
MH  - Trigeminal Ganglion/*drug effects/metabolism
OTO - NOTNLM
OT  - Calcium imaging
OT  - Endothelin
OT  - Gap junctions
OT  - Nodose ganglion
OT  - Satellite glial cells
OT  - Superior cervical ganglion
OT  - Sympathetic ganglia
OT  - Trigeminal ganglion
EDAT- 2017/03/28 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/03/27 06:00
PHST- 2016/11/17 00:00 [received]
PHST- 2017/03/01 00:00 [revised]
PHST- 2017/03/16 00:00 [accepted]
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/03/27 06:00 [entrez]
AID - S0143-4179(16)30176-7 [pii]
AID - 10.1016/j.npep.2017.03.002 [doi]
PST - ppublish
SO  - Neuropeptides. 2017 Jun;63:37-42. doi: 10.1016/j.npep.2017.03.002. Epub 2017 Mar 
      18.