PMID- 28343338
OWN - NLM
STAT- MEDLINE
DCOM- 20180202
LR  - 20181202
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 48
IP  - 3
DP  - 2017 Jun
TI  - Expression of typical osteoclast markers by PBMCs after PEG-induced fusion as a 
      model for studying osteoclast differentiation.
PG  - 169-185
LID - 10.1007/s10735-017-9717-4 [doi]
AB  - Bone is a metabolically active organ subjected to continuous remodeling process 
      that involves resorption by osteoclast and subsequent formation by osteoblasts. 
      Osteoclast involvement in this physiological event is regulated by macrophage 
      colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappaB 
      ligand (RANKL). Fusion of mono-nuclear pre-osteoclasts is a critical event for 
      osteoclast differentiation and for bone resorption. Here we show that PBMCs can 
      be successfully fused with polyethylenglicol (PEG) in order to generated viable 
      osteoclast-like cells that exhibit tartrate-resistant acid phosphatase (TRAP) and 
      bone resorptive activities. PEG-fused PBMCs expressed additional markers 
      compatible with osteoclastogenic differentiation such as carbonic anhydrase II 
      (CAII), calcitonin receptor (CR), cathepsin K (Cat K), vacuolar ATPase (V-ATPase) 
      subunit C1 (V-ATPase), integrin beta3, RANK and cell surface aminopeptidase N/CD13. 
      Actin redistribution in PEG-fused cells was found to be affected by cell cycle 
      synchronization at G0/G1 or G2/M phases. PEG-induced fusion also led to 
      expression of tyrosine kinases c-Src and Syk in their phosphorylated state. 
      Scanning electron microscopy images showed morphological features typical of 
      osteoclast-like cells. The results here shown allow concluding that PEG-induced 
      fusion of PBMCs provides a suitable model system for understanding the mechanisms 
      involved in osteoclastogenesis and for assaying new therapeutic strategies.
FAU - Castillo, Luz M
AU  - Castillo LM
AD  - Departamento de Ciencias Fisiologicas, Facultad de Medicina, Universidad Nacional 
      de Colombia, Bogota, Colombia.
FAU - Guerrero, Carlos A
AU  - Guerrero CA
AD  - Departamento de Ciencias Fisiologicas, Facultad de Medicina, Universidad Nacional 
      de Colombia, Bogota, Colombia. caguerrerof@unal.edu.co.
FAU - Acosta, Orlando
AU  - Acosta O
AD  - Departamento de Ciencias Fisiologicas, Facultad de Medicina, Universidad Nacional 
      de Colombia, Bogota, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20170325
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Biomarkers)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - EC 3.1.3.2 (ACP5 protein, human)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Biomarkers/analysis/blood
MH  - Bone Resorption
MH  - *Cell Differentiation
MH  - *Cell Fusion
MH  - Cells, Cultured
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/*metabolism
MH  - Models, Biological
MH  - Osteoclasts/*cytology
MH  - Polyethylene Glycols
MH  - Tartrate-Resistant Acid Phosphatase/metabolism
OTO - NOTNLM
OT  - Bone resorption
OT  - Osteoclast activity
OT  - Osteoclast differentiation
OT  - Peripheral blood mononuclear cells
OT  - Polyethylenglicol
EDAT- 2017/03/28 06:00
MHDA- 2018/02/03 06:00
CRDT- 2017/03/27 06:00
PHST- 2016/11/17 00:00 [received]
PHST- 2017/03/20 00:00 [accepted]
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2018/02/03 06:00 [medline]
PHST- 2017/03/27 06:00 [entrez]
AID - 10.1007/s10735-017-9717-4 [pii]
AID - 10.1007/s10735-017-9717-4 [doi]
PST - ppublish
SO  - J Mol Histol. 2017 Jun;48(3):169-185. doi: 10.1007/s10735-017-9717-4. Epub 2017 
      Mar 25.