PMID- 28343378
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 40
IP  - 3
DP  - 2017 Mar
TI  - Adipose-Derived Stem Cells Suppress Inflammation Induced by IL-1beta through 
      Down-Regulation of P2X7R Mediated by miR-373 in Chondrocytes of Osteoarthritis.
PG  - 222-229
LID - 10.14348/molcells.2017.2314 [doi]
AB  - Adipose-derived stem cells (ADSCs) were previously considered to have an 
      anti-inflammatory effect, and Interleukin-1beta (IL-1beta) was found to be a 
      pro-inflammatory factor in chondrocytes, but the mechanism underlying ADSCs and 
      IL-1beta is unclear. In this study, we investigate whether P2X7 receptor (P2X7R) 
      signalling, regulated by microRNA 373 (miR-373), was involved in the ADSCs and 
      IL-1beta mediated inflammation in osteoarthritis (OA). Chondrocytes were collected 
      from 20 OA patients and 20 control participants, and ADSCs were collected from 
      patients who had undergone abdominal surgery. The typical surface molecules of 
      ASDCs were detected by flow cytometry. The level of nitric oxide (NO) was 
      determined by Griess reagent. Concentrations of prostaglandin E2 (PGE2), 
      interleukin 6 (IL-6), matrix metallopeptidase 3 (MMP-3) were detected by 
      enzyme-linked immunosorbent assay (ELISA). The expressions of IL-6, MMP-3, 
      miR-373 and P2X7R were determined by real-time polymerase chain reaction (PCR), 
      and Western blot was used to detect the protein expression of P2X7R. The typical 
      potential characters of ADSCs were verified. In chondrocytes or OA tissues, the 
      miR-373 expression level was decreased, but the P2X7R expression was increased. 
      IL-1beta stimulation increased the level of inflammatory factors in OA chondrocytes, 
      and ADSCs co-cultured with IL-1beta-stimulated chondrocytes decreased the 
      inflammation. OA chondrocytes transfected with the miR-373 inhibitor increased 
      the inflammation level. The miR-373 mimic suppressed the inflammation by 
      targeting P2X7R and regulated its expression, while its effect was reversed by 
      overexpression of P2X7R. IL-1beta induced inflammation in OA chondrocytes, while 
      ADSCs seemed to inhibit the expression of P2X7R that was regulated by miR-373 and 
      involved in the anti-inflammatory process in OA.
FAU - Jin, Rilong
AU  - Jin R
AD  - Department of Orthopedics Surgery, The First Affiliated Hospital, College of 
      Medical Zhejiang University, Hangzhou, 310003 China.
FAU - Shen, Miaoda
AU  - Shen M
AD  - Department of Orthopedics Surgery, The First Affiliated Hospital, College of 
      Medical Zhejiang University, Hangzhou, 310003 China.
FAU - Yu, Liedao
AU  - Yu L
AD  - Department of Orthopedics Surgery, The First Affiliated Hospital, College of 
      Medical Zhejiang University, Hangzhou, 310003 China.
FAU - Wang, Xuanwei
AU  - Wang X
AD  - Department of Orthopedics Surgery, The First Affiliated Hospital, College of 
      Medical Zhejiang University, Hangzhou, 310003 China.
FAU - Lin, Xiangjin
AU  - Lin X
AD  - Department of Orthopedics Surgery, The First Affiliated Hospital, College of 
      Medical Zhejiang University, Hangzhou, 310003 China.
LA  - eng
PT  - Journal Article
DEP - 20170327
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN374 microRNA 374, human)
RN  - 0 (MicroRNAs)
RN  - 0 (P2RX7 protein, human)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adipocytes/*cytology/metabolism
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/metabolism
MH  - Coculture Techniques
MH  - Dinoprostone/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-1beta/*pharmacology
MH  - Interleukin-6/genetics/metabolism
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - MicroRNAs/*genetics
MH  - Nitric Oxide/metabolism
MH  - Osteoarthritis/genetics/*immunology
MH  - Receptors, Purinergic P2X7/*genetics/metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology/*metabolism
PMC - PMC5386960
OTO - NOTNLM
OT  - P2X7R
OT  - adipose-derived stem cells
OT  - chondrocytes
OT  - miR-373
OT  - osteoarthritis
EDAT- 2017/03/28 06:00
MHDA- 2017/05/06 06:00
PMCR- 2017/03/27
CRDT- 2017/03/28 06:00
PHST- 2016/12/26 00:00 [received]
PHST- 2017/01/17 00:00 [revised]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2017/03/28 06:00 [entrez]
PHST- 2017/03/27 00:00 [pmc-release]
AID - S1016-8478(23)00605-2 [pii]
AID - molce-40-3-222 [pii]
AID - 10.14348/molcells.2017.2314 [doi]
PST - ppublish
SO  - Mol Cells. 2017 Mar;40(3):222-229. doi: 10.14348/molcells.2017.2314. Epub 2017 
      Mar 27.