PMID- 28346809
OWN - NLM
STAT- MEDLINE
DCOM- 20170619
LR  - 20220408
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 16
IP  - 6
DP  - 2017 Mar 19
TI  - MALAT1 promotes osteosarcoma development by regulation of HMGB1 via miR-142-3p 
      and miR-129-5p.
PG  - 578-587
LID - 10.1080/15384101.2017.1288324 [doi]
AB  - Recently, emerging evidence has demonstrated that metastasis-associated lung 
      adenocarcinoma transcript 1 (MALAT1), a long non-coding RNAs (lncRNAs), 
      contributes to the initiation and development of tumors, including osteosarcoma 
      (OS). Multiple studies have suggested an oncogenic role of MALAT1 and 
      high-mobility group protein B1 (HMGB1) in OS tumorigenesis and metastasis, but 
      the effects and mechanisms are not unanimous. Here, we showed that MALAT1 and 
      HMGB1 were significantly increased in human OS cell lines and knockdown of MALAT1 
      reduced HMGB1 expression. By using online tools, we screen out 2 candidate 
      miRNAs, miR-142-3p and miR-129-5p which may be associated with both MALAT1 and 
      HMGB1. Luciferase reporter assay revealed a direct interaction between the 2 
      miRNAs and MALAT1, respectively, via a putative binding site within MALAT1. 
      Meanwhile, both the 2 miRNAs could bind to HMGB1 3'-untranslated region (3'-UTR) 
      and regulate HMGB1 expression. Moreover, knockdown of MALAT1 decreased HMGB1 
      expression, inhibited OS cell growth and promoted apoptosis, while miR-142-3p and 
      miR-129-5p inhibitor partly restored the inhibitory effect of MALAT1 knockdown on 
      HMGB1 expression, OS cell growth and the promotion of apoptosis. In OS tissues, 
      the expression of MALAT1 and HMGB1 was upregulated while the expression of 
      miR-142-3p and miR-129-5p was downregulated. Together, our results support a 
      MALAT1/miR-142-3p/miR-129-5p/HMGB1 axis in OS cell proliferation and tumor 
      progression. MALAT1 promoted OS cell growth through inhibition of miR-142-3p or 
      miR-129-5p and by targeting HMGB1.
FAU - Liu, Ke
AU  - Liu K
AD  - a Department of Ophthalmology , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Huang, Jun
AU  - Huang J
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Ni, Jiangdong
AU  - Ni J
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Song, Deye
AU  - Song D
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Ding, Muliang
AU  - Ding M
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Wang, Junjie
AU  - Wang J
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Huang, Xianzhe
AU  - Huang X
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
FAU - Li, Wenzhao
AU  - Li W
AD  - b Department of Orthopaedics , The Second Xiangya Hospital, Central South 
      University , Changsha , Hunan , P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170210
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HMGB1 Protein)
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (MIRN142 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Apoptosis/genetics
MH  - Base Sequence
MH  - Bone Neoplasms/*pathology
MH  - Carcinogenesis/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - HMGB1 Protein/*genetics/metabolism
MH  - Humans
MH  - MicroRNAs/*genetics/metabolism
MH  - Osteosarcoma/*genetics/*pathology
MH  - RNA, Long Noncoding/genetics/*metabolism
PMC - PMC5384591
OTO - NOTNLM
OT  - HMGB1
OT  - lncRNA-MALAT1
OT  - miR-129-5p
OT  - miR-142-3p
OT  - osteosarcoma
EDAT- 2017/03/28 06:00
MHDA- 2017/06/20 06:00
PMCR- 2018/02/10
CRDT- 2017/03/28 06:00
PHST- 2017/03/28 06:00 [entrez]
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2017/06/20 06:00 [medline]
PHST- 2018/02/10 00:00 [pmc-release]
AID - 1288324 [pii]
AID - 10.1080/15384101.2017.1288324 [doi]
PST - ppublish
SO  - Cell Cycle. 2017 Mar 19;16(6):578-587. doi: 10.1080/15384101.2017.1288324. Epub 
      2017 Feb 10.