PMID- 28346830
OWN - NLM
STAT- MEDLINE
DCOM- 20170908
LR  - 20170908
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 36
IP  - 6
DP  - 2017 Jun
TI  - Synergetic Neuroprotective Effect of Docosahexaenoic Acid and Aspirin in SH-Y5Y 
      by Inhibiting miR-21 and Activating RXRalpha and PPARalpha.
PG  - 482-489
LID - 10.1089/dna.2017.3643 [doi]
AB  - Parkinson's disease (PD) is a serious neurodegenerative disorder that lacks 
      effective therapeutic methods. In this research, expressions of PPARalpha, RXRalpha, and 
      miR-21 were evaluated in PD patients and normal controls. To investigate the 
      effects of miR-21, docosahexaenoic acid (DHA) and aspirin (ASA) on PD, as well as 
      the relationships between them, SH-Y5Y cells were treated with DHA, ASA, or both 
      for 24 h. The assay showed that levels of miR-21 were increased and levels of 
      PPARalpha were decreased in PD patients compared with normal controls. miR-21 was 
      negatively correlated with PPARalpha in PD patients. DHA and ASA could activate RXRalpha 
      and PPARalpha, respectively. Additionally, DHA upregulated PPARalpha expression by 
      inhibiting miR-21 in SH-Y5Y cells. A combination of DHA and ASA efficiently 
      enhanced heterodimer formations of PPARalpha and RXRalpha and increased the expression of 
      neurotrophic factors PSD-95, brain-derived neurotrophic factor (BDNF), and glial 
      cell-derived neurotrophic factor (GDNF), while inhibiting NFkappaB and COX2. These 
      findings suggest that a combination of DHA and ASA could significantly improve 
      the expression of PSD-95, BDNF, and GDNF by promoting heterodimerization of PPARalpha 
      and RXRalpha, thus supplying a new therapeutic method for PD.
FAU - Fu, Yongwang
AU  - Fu Y
AD  - 1 Department of Neurology, Renmin Hospital of Wuhan University, Wuhan University 
      , Wuhan, China .
FAU - Zhen, Jin
AU  - Zhen J
AD  - 2 Department of Neurology, Inner Mongolia Autonomous Region People's Hospital , 
      Hohhot, China .
FAU - Lu, Zuneng
AU  - Lu Z
AD  - 1 Department of Neurology, Renmin Hospital of Wuhan University, Wuhan University 
      , Wuhan, China .
LA  - eng
PT  - Journal Article
DEP - 20170327
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (PPAR alpha)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Aspirin/*pharmacology
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Docosahexaenoic Acids/*pharmacology
MH  - Drug Synergism
MH  - Humans
MH  - MicroRNAs/*antagonists & inhibitors
MH  - Neuroprotective Agents/*pharmacology
MH  - PPAR alpha/agonists/genetics/*metabolism
MH  - Retinoid X Receptor alpha/agonists/*metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - ASA
OT  - DHA
OT  - PPARalpha
OT  - Parkinson's disease
OT  - RXRalpha
OT  - miR-21
EDAT- 2017/03/28 06:00
MHDA- 2017/09/09 06:00
CRDT- 2017/03/28 06:00
PHST- 2017/03/28 06:00 [pubmed]
PHST- 2017/09/09 06:00 [medline]
PHST- 2017/03/28 06:00 [entrez]
AID - 10.1089/dna.2017.3643 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2017 Jun;36(6):482-489. doi: 10.1089/dna.2017.3643. Epub 2017 Mar 
      27.