PMID- 28347639
OWN - NLM
STAT- MEDLINE
DCOM- 20171212
LR  - 20180309
IS  - 1872-7484 (Electronic)
IS  - 1566-0702 (Linking)
VI  - 205
DP  - 2017 Jul
TI  - Neurite outgrowth in cultured mouse pelvic ganglia - Effects of neurotrophins and 
      bladder tissue.
PG  - 41-49
LID - S1566-0702(16)30185-0 [pii]
LID - 10.1016/j.autneu.2017.03.004 [doi]
AB  - Neurotrophic factors regulate survival and growth of neurons. The urinary bladder 
      is innervated via both sympathetic and parasympathetic neurons located in the 
      major pelvic ganglion. The aim of the present study was to characterize the 
      effects of the neurotrophins nerve growth factor (NGF), brain derived 
      neurotrophic factor (BDNF) and neurotrophin 3 (NT-3) on the sprouting rate of 
      sympathetic and parasympathetic neurites from the female mouse ganglion. The 
      pelvic ganglion was dissected out and attached to a petri dish and cultured in 
      vitro. All three factors (BDNF, NT-3 and NGF) stimulated neurite outgrowth of 
      both sympathetic and parasympathetic neurites although BDNF and NT-3 had a higher 
      stimulatory effect on parasympathetic ganglion cells. The neurotrophin receptors 
      TrkA, TrkB and TrkC were all expressed in neurons of the ganglia. Co-culture of 
      ganglia with urinary bladder tissue, but not diaphragm tissue, increased the 
      sprouting rate of neurites. Active forms of BDNF and NT-3 were detected in 
      urinary bladder tissue using western blotting whereas tissue from the diaphragm 
      expressed NGF. Neurite outgrowth from the pelvic ganglion was inhibited by a TrkB 
      receptor antagonist. We therefore suggest that the urinary bladder releases 
      trophic factors, including BDNF and NT-3, which regulate neurite outgrowth via 
      activation of neuronal Trk-receptors. These findings could influence future 
      strategies for developing pharmaceuticals to improve re-innervation due to 
      bladder pathologies.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Ekman, Mari
AU  - Ekman M
AD  - Department of Experimental Medical Science, Lund University, Lund, Sweden; 
      Department of Biology, Lund University, Lund, Sweden. Electronic address: 
      mari.ekman@med.lu.se.
FAU - Zhu, Baoyi
AU  - Zhu B
AD  - Department of Experimental Medical Science, Lund University, Lund, Sweden; 
      Department of Urology, The Sixth Affiliated Hospital of Guangzhou Medical 
      University, Guang Dong, China.
FAU - Sward, Karl
AU  - Sward K
AD  - Department of Experimental Medical Science, Lund University, Lund, Sweden.
FAU - Uvelius, Bengt
AU  - Uvelius B
AD  - Department of Urology, Clinical Sciences, Lund University, Lund, Sweden.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170318
PL  - Netherlands
TA  - Auton Neurosci
JT  - Autonomic neuroscience : basic & clinical
JID - 100909359
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/*metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Diaphragm/innervation
MH  - Female
MH  - Ganglia, Autonomic/cytology/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Nerve Growth Factor/administration & dosage/*metabolism
MH  - Neuronal Outgrowth/drug effects/*physiology
MH  - Neurotrophin 3/administration & dosage/*metabolism
MH  - Parasympathetic Nervous System/cytology/drug effects/metabolism
MH  - Pelvis
MH  - Prostate/innervation
MH  - Receptor Protein-Tyrosine Kinases/antagonists & inhibitors/metabolism
MH  - Sympathetic Nervous System/cytology/drug effects/metabolism
MH  - Urinary Bladder/*innervation
OTO - NOTNLM
OT  - BNDF
OT  - NGF
OT  - NT-3
OT  - Parasympathetic
OT  - Sympathetic
OT  - Trk receptors
EDAT- 2017/03/30 06:00
MHDA- 2017/12/13 06:00
CRDT- 2017/03/29 06:00
PHST- 2016/09/16 00:00 [received]
PHST- 2017/03/14 00:00 [revised]
PHST- 2017/03/17 00:00 [accepted]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/12/13 06:00 [medline]
PHST- 2017/03/29 06:00 [entrez]
AID - S1566-0702(16)30185-0 [pii]
AID - 10.1016/j.autneu.2017.03.004 [doi]
PST - ppublish
SO  - Auton Neurosci. 2017 Jul;205:41-49. doi: 10.1016/j.autneu.2017.03.004. Epub 2017 
      Mar 18.