PMID- 28347764
OWN - NLM
STAT- MEDLINE
DCOM- 20170817
LR  - 20190107
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 293
DP  - 2017 Jul
TI  - BDNF is required for taste axon regeneration following unilateral chorda tympani 
      nerve section.
PG  - 27-42
LID - S0014-4886(17)30076-6 [pii]
LID - 10.1016/j.expneurol.2017.03.016 [doi]
AB  - Taste nerves readily regenerate to reinnervate denervated taste buds; however, 
      factors required for regeneration have not yet been identified. When the chorda 
      tympani nerve is sectioned, expression of brain-derived neurotrophic factor 
      (BDNF) remains high in the geniculate ganglion and lingual epithelium, despite 
      the loss of taste buds. These observations suggest that BDNF is present in the 
      taste system after nerve section and may support taste nerve regeneration. To 
      test this hypothesis, we inducibly deleted Bdnf during adulthood in mice. Shortly 
      after Bdnf gene recombination, the chorda tympani nerve was unilaterally 
      sectioned causing a loss of both taste buds and neurons, irrespective of BDNF 
      levels. Eight weeks after nerve section, however, regeneration was differentially 
      affected by Bdnf deletion. In control mice, there was regeneration of the chorda 
      tympani nerve and taste buds reappeared with innervation. In contrast, few taste 
      buds were reinnervated in mice lacking normal Bdnf expression such that taste bud 
      number remained low. In all genotypes, taste buds that were reinnervated were 
      normal-sized, but non-innervated taste buds remained small and atrophic. On the 
      side of the tongue contralateral to the nerve section, taste buds for some 
      genotypes became larger and all taste buds remained innervated. Our findings 
      suggest that BDNF is required for nerve regeneration following gustatory nerve 
      section.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Meng, Lingbin
AU  - Meng L
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY 40292, USA.
FAU - Huang, Tao
AU  - Huang T
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY 40292, USA.
FAU - Sun, Chengsan
AU  - Sun C
AD  - Department of Psychology, University of Virginia, Charlottesville, VA 22904, USA.
FAU - Hill, David L
AU  - Hill DL
AD  - Department of Psychology, University of Virginia, Charlottesville, VA 22904, USA.
FAU - Krimm, Robin
AU  - Krimm R
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville 
      School of Medicine, Louisville, KY 40292, USA. Electronic address: 
      rfkrim01@louisville.edu.
LA  - eng
GR  - R01 DC000407/DC/NIDCD NIH HHS/United States
GR  - R01 DC006938/DC/NIDCD NIH HHS/United States
GR  - R01 DC007176/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170325
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Tubulin)
RN  - 0 (beta3 tubulin, mouse)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Chorda Tympani Nerve/*injuries
MH  - Disease Models, Animal
MH  - Estrogen Antagonists/pharmacology
MH  - Facial Nerve Diseases/*pathology
MH  - Female
MH  - Functional Laterality/*physiology
MH  - Gene Expression Regulation/drug effects/genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Nerve Regeneration/*physiology
MH  - Tamoxifen/pharmacology
MH  - Taste/*physiology
MH  - Taste Buds/pathology
MH  - Time Factors
MH  - Tubulin/metabolism
MH  - beta-Galactosidase/metabolism
PMC - PMC5625336
MID - NIHMS864208
OTO - NOTNLM
OT  - BDNF
OT  - Nerve regeneration
OT  - Neurotrophins
OT  - Sensory
OT  - Taste
OT  - Taste bud
EDAT- 2017/03/30 06:00
MHDA- 2017/08/18 06:00
PMCR- 2018/07/01
CRDT- 2017/03/29 06:00
PHST- 2016/11/15 00:00 [received]
PHST- 2017/03/22 00:00 [revised]
PHST- 2017/03/23 00:00 [accepted]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
PHST- 2017/03/29 06:00 [entrez]
PHST- 2018/07/01 00:00 [pmc-release]
AID - S0014-4886(17)30076-6 [pii]
AID - 10.1016/j.expneurol.2017.03.016 [doi]
PST - ppublish
SO  - Exp Neurol. 2017 Jul;293:27-42. doi: 10.1016/j.expneurol.2017.03.016. Epub 2017 
      Mar 25.