PMID- 28347884
OWN - NLM
STAT- MEDLINE
DCOM- 20170705
LR  - 20200407
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 506
DP  - 2017 Jun
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 
      expression through TAK-1/JNK/AP-1 pathways.
PG  - 64-72
LID - S0042-6822(17)30092-2 [pii]
LID - 10.1016/j.virol.2017.03.009 [doi]
AB  - The acute phase of respiratory distress caused by porcine reproductive and 
      respiratory syndrome virus (PRRSV) is likely a consequence of the release of 
      inflammatory cytokines in the lung. IL-8, the main chemokine and activator of 
      neutrophils, might be related to the lung injury upon PRRSV infection. In this 
      study, we showed that PRRSV induced IL-8 expression in vivo and in vitro. 
      Subsequently, we demonstrated that JNK and NF-kappaB pathways were activated upon 
      PRRSV infection and required for the enhancement of IL-8 expression. We further 
      verified that PRRSV-activated TAK-1 was essential for the activation of JNK and 
      NF-kappaB pathways and IL-8 expression. Moreover, we revealed an AP-1 binding motif 
      in the cloned porcine IL-8 (pIL-8) promoter, and deletion of this motif abolished 
      the pIL-8 promoter activity. Finally, we found that the JNK-activated AP-1 
      subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV. 
      These data suggest that PRRSV-induced IL-8 production is likely through the 
      TAK-1/JNK/AP-1 pathways.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Liu, Yihao
AU  - Liu Y
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Du, Yinping
AU  - Du Y
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Wang, Honglei
AU  - Wang H
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Du, Li
AU  - Du L
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Feng, Wen-Hai
AU  - Feng WH
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China. Electronic address: whfeng@cau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170324
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Interleukin-8)
RN  - 0 (Transcription Factor AP-1)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (MAP kinase kinase kinase 7)
SB  - IM
MH  - Animals
MH  - Interleukin-8/*genetics/immunology
MH  - JNK Mitogen-Activated Protein Kinases/genetics/*metabolism
MH  - MAP Kinase Kinase Kinases/genetics/*metabolism
MH  - Porcine Reproductive and Respiratory 
      Syndrome/enzymology/*genetics/metabolism/virology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Signal Transduction
MH  - Swine
MH  - Transcription Factor AP-1/genetics/*metabolism
MH  - Up-Regulation
PMC - PMC7111726
OTO - NOTNLM
OT  - AP-1
OT  - IL-8
OT  - JNK
OT  - PRRSV
OT  - TAK-1
EDAT- 2017/03/30 06:00
MHDA- 2017/07/06 06:00
PMCR- 2017/03/24
CRDT- 2017/03/29 06:00
PHST- 2016/12/08 00:00 [received]
PHST- 2017/03/08 00:00 [revised]
PHST- 2017/03/21 00:00 [accepted]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/07/06 06:00 [medline]
PHST- 2017/03/29 06:00 [entrez]
PHST- 2017/03/24 00:00 [pmc-release]
AID - S0042-6822(17)30092-2 [pii]
AID - 10.1016/j.virol.2017.03.009 [doi]
PST - ppublish
SO  - Virology. 2017 Jun;506:64-72. doi: 10.1016/j.virol.2017.03.009. Epub 2017 Mar 24.