PMID- 28350079
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210427
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 50
IP  - 4
DP  - 2017 Apr
TI  - Effects of irradiation on human leukocyte antigen class I expression in human 
      papillomavirus positive and negative base of tongue and mobile tongue squamous 
      cell carcinoma cell lines.
PG  - 1423-1430
LID - 10.3892/ijo.2017.3916 [doi]
AB  - Human papillomavirus (HPV) infection is a risk factor for oropharyngeal cancer, 
      besides smoking and alcohol. Patients with HPV-positive tumors have a better 
      prognosis than those with HPV-negative tumors. Furthermore, patients with 
      HPV-positive tumors, with high CD8+ tumor infiltrating lymphocyte counts or 
      absent/low human leukocyte antigen (HLA) class I expression have the best 
      outcome. The latter is paradoxical, since HLA class I expression is important for 
      tumor recognition. Below, the hypothesis that radiation therapy increases HLA 
      class I expression was tested. HPV16 positive head and neck cancer cell lines 
      UPCI-SCC-154, UPCI-SCC-090 and UM-SCC-47, and the HPV-negative cancer cell line 
      UT-SCC-14, were treated with 2-10 Gray (Gy) and tested for HLA class I 
      expression, cell cycle changes and apoptosis by flow cytometry. HPV16 E5, E7 and 
      HLA-A mRNA expression was tested by quantitative PCR. A dose of 10 Gy resulted in 
      a tendency of increased HLA class I cell surface expression for all cell lines 
      and reached statistical significance for UPCI-SCC-154 and UPCI-SCC-090. There 
      were, however, no significant changes in HLA-A mRNA expression in any of the cell 
      lines, or HPV16 E5, or E7 mRNA expression for UPCI-SCC-47 and UPCI-SCC-154, while 
      for UPCI-SCC-090 HPV16 E5 mRNA decreased. In all cell lines there was a shift 
      towards G2/M phase and increased apoptosis after irradiation with 10 Gy. To 
      conclude, irradiation with 10 Gy increased HLA class I expression in the 
      HPV-positive cell lines UPCI-SCC-154 and UPCI-SCC-090. A similar tendency was 
      observed for HPV-positive UM-SCC-47 and HPV-negative UT-SCC-14.
FAU - Haeggblom, Linnea
AU  - Haeggblom L
AD  - Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska 
      (CCK), Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Nordfors, Cecilia
AU  - Nordfors C
AD  - Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 
      171 77 Stockholm, Sweden.
FAU - Tertipis, Nikolaos
AU  - Tertipis N
AD  - Department of Medical Epidemiology and Biostatistics (MEB), Karolinska 
      Institutet, 171 77 Stockholm, Sweden.
FAU - Bersani, Cinzia
AU  - Bersani C
AD  - Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska 
      (CCK), Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Ramqvist, Torbjorn
AU  - Ramqvist T
AD  - Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska 
      (CCK), Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Nasman, Anders
AU  - Nasman A
AD  - Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska 
      (CCK), Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Dalianis, Tina
AU  - Dalianis T
AD  - Department of Oncology-Pathology, Karolinska Institutet, Cancer Center Karolinska 
      (CCK), Karolinska University Hospital, 171 76 Stockholm, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20170316
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
EDAT- 2017/03/30 06:00
MHDA- 2017/03/30 06:01
CRDT- 2017/03/29 06:00
PHST- 2016/12/12 00:00 [received]
PHST- 2017/02/06 00:00 [accepted]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/03/30 06:01 [medline]
PHST- 2017/03/29 06:00 [entrez]
AID - 10.3892/ijo.2017.3916 [doi]
PST - ppublish
SO  - Int J Oncol. 2017 Apr;50(4):1423-1430. doi: 10.3892/ijo.2017.3916. Epub 2017 Mar 
      16.