PMID- 28351209
OWN - NLM
STAT- MEDLINE
DCOM- 20180622
LR  - 20190202
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 34
IP  - 21
DP  - 2017 Nov 1
TI  - Effects of Multiple Injection of Bone Marrow Mononuclear Cells on Spinal Cord 
      Injury of Rats.
PG  - 3003-3011
LID - 10.1089/neu.2016.4841 [doi]
AB  - The effects of multiple injection of bone marrow mononuclear cells (BMNCs) on 
      spinal cord injury (SCI) were compared with those of single injection in rats. 
      BMNCs separated by density-gradient centrifugation from a bone marrow perfusate 
      were injected three times (once weekly) through the cerebrospinal fluid (CSF) via 
      the fourth ventricle, and the locomotor improvement and tissue recovery, 
      including axonal regeneration, were compared with those of single injection. 
      While the single-injection group showed a steep elevation of the 
      Basso-Beattie-Bresnahan (BBB) score 1 week after transplantation, the 
      multiple-injection group maintained a similar steep elevation for 2 weeks after 
      transplantation, and the BBB scores of the multiple-injection group remained 
      thereafter at a level approximately 2-3 points higher than those of the 
      single-injection group until the end of the experiment. There were significant 
      differences between the single- and multiple-injection groups at 3, 4, and 8 
      weeks after transplantation. The difference in BBB scores at 8 weeks after 
      transplantation suggested that there was a marked difference in the quality of 
      locomotor behaviors between the single-and multiple-injection groups at this 
      stage. An extensive outgrowth of regenerating axons through the astrocyte-devoid 
      areas and a marked reduction of cavity formation were found in both the single- 
      and multiple-injection groups. There were, however, no significant differences in 
      the density of regenerating axons or volumes of cavities between the single- and 
      multiple-injection groups. These results showed that although tissue recoveries 
      were similar between single and multiple injection, the multiple injection of 
      BMNCs was more beneficial for locomotor improvement than single injection for the 
      treatment of SCI. Considering the technically simple and low-cost procedures for 
      the preparation and injection of BMNCs, multiple injection of BMNCs by lumbar 
      puncture has an advantage over single injection on clinical application.
FAU - Kanekiyo, Kenji
AU  - Kanekiyo K
AD  - 1 The Central Biomedical Laboratory, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
FAU - Nakano, Norihiko
AU  - Nakano N
AD  - 1 The Central Biomedical Laboratory, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
FAU - Homma, Tamami
AU  - Homma T
AD  - 1 The Central Biomedical Laboratory, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
FAU - Yamada, Yoshihiro
AU  - Yamada Y
AD  - 2 Department of Physical Therapy, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
FAU - Tamachi, Masahiro
AU  - Tamachi M
AD  - 2 Department of Physical Therapy, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
FAU - Suzuki, Yoshihisa
AU  - Suzuki Y
AD  - 3 Department of Plastic and Reconstructive Surgery, Tazuke Medical Research 
      Institute , Kitano Hospital, Osaka City, Osaka, Japan .
FAU - Fukushima, Masanori
AU  - Fukushima M
AD  - 4 Translational Research Informatics Center , Foundation for Biomedical Research 
      and Innovation, Kobe City, Hyogo Prefecture, Japan .
FAU - Saito, Fukuki
AU  - Saito F
AD  - 5 Emergency and Clinical Care Center, Kansai Medical University , Hirakata City, 
      Osaka, Japan .
FAU - Ide, Chizuka
AU  - Ide C
AD  - 1 The Central Biomedical Laboratory, Aino University School of Health Science , 
      Ibaraki City, Osaka, Japan .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170629
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
SB  - IM
MH  - Animals
MH  - Female
MH  - Leukocytes, Mononuclear/*transplantation
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Nerve Regeneration
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Spinal Cord Injuries/*pathology
OTO - NOTNLM
OT  - axonal regeneration
OT  - bone marrow mononuclear cell
OT  - bone marrow stromal cells
OT  - locomotor improvement
OT  - multiple-injection
OT  - spinal cord injury
EDAT- 2017/03/30 06:00
MHDA- 2018/06/23 06:00
CRDT- 2017/03/30 06:00
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2018/06/23 06:00 [medline]
PHST- 2017/03/30 06:00 [entrez]
AID - 10.1089/neu.2016.4841 [doi]
PST - ppublish
SO  - J Neurotrauma. 2017 Nov 1;34(21):3003-3011. doi: 10.1089/neu.2016.4841. Epub 2017 
      Jun 29.