PMID- 28352316
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 13
IP  - 2
DP  - 2017 Feb
TI  - Monocyte chemoattractant protein-1 contributes to morphine tolerance in rats with 
      cancer-induced bone pain.
PG  - 461-466
LID - 10.3892/etm.2016.3979 [doi]
AB  - Cancer-induced bone pain can severely compromise the life quality of patients, 
      while tolerance limits the use of opioids in the treatment of cancer pain. 
      Monocyte chemoattractant protein-1 (MCP-1) is known to contribute to neuropathic 
      pain. However, the role of spinal MCP-1 in the development of morphine tolerance 
      in patients with cancer-induced bone pain remains unclear. The aim of the present 
      study was to investigate the role of spinal MCP-1 in morphine tolerance in bone 
      cancer pain rats (MTBP rats). Bone cancer pain was induced by intramedullary 
      injection of Walker 256 cells into the tibia of the rats, while morphine 
      tolerance was induced by continuous intrathecal injection of morphine over a 
      period of 9 days. In addition, anti-MCP-1 antibodies were intrathecally injected 
      to rats in various groups in order to investigate the association of MCP-1 with 
      mechanical and heat hyperalgesia using the paw withdrawal threshold (PWT) and 
      thermal withdrawal latency (TWL) tests, respectively. Furthermore, MCP-1 and CCR2 
      expression levels were measured using reverse transcription-quantitative 
      polymerase chain reaction (RT-qPCR) and western blot analysis, and CCR2 
      expression levels were measured using RT-qPCR. The results indicated that MCP-1 
      and CCR2 expression levels were significantly increased in the spinal cord of 
      MTBP rats. Intrathecal administration of anti-MCP-1 neutralizing antibodies was 
      observed to attenuate the mechanical and thermal allodynia in MTBP rats. 
      Therefore, the upregulation of spinal MCP-1 and CCR2 expression levels may 
      contribute to the development of mechanical allodynia in MTBP rats. In 
      conclusion, MCP-1/CCR2 signaling may serve a crucial role in morphine tolerance 
      development in rats suffering from cancer-induced bone pain.
FAU - Liu, Lei
AU  - Liu L
AD  - Department of Pain Management, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong 250021, P.R. China; Department of 
      Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. 
      China.
FAU - Gao, Xiu-Juan
AU  - Gao XJ
AD  - Department of Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 
      252000, P.R. China.
FAU - Ren, Chun-Guang
AU  - Ren CG
AD  - Department of Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 
      252000, P.R. China.
FAU - Hu, Ji-Hua
AU  - Hu JH
AD  - Department of Anesthesiology, The First Affiliated Hospital of Soochow 
      University, Suzhou, Jiangsu 215006, P.R. China.
FAU - Liu, Xian-Wen
AU  - Liu XW
AD  - Department of Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 
      252000, P.R. China.
FAU - Zhang, Ping
AU  - Zhang P
AD  - Department of Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 
      252000, P.R. China.
FAU - Zhang, Zong-Wang
AU  - Zhang ZW
AD  - Department of Anesthesiology, Liaocheng People's Hospital, Liaocheng, Shandong 
      252000, P.R. China.
FAU - Fu, Zhi-Jian
AU  - Fu ZJ
AD  - Department of Pain Management, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong 250021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20161216
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC5348680
OTO - NOTNLM
OT  - C-C chemokine receptor type 2
OT  - bone cancer pain
OT  - monocyte chemoattractant protein-1
OT  - morphine tolerance
OT  - spinal cord
EDAT- 2017/03/30 06:00
MHDA- 2017/03/30 06:01
PMCR- 2016/12/16
CRDT- 2017/03/30 06:00
PHST- 2015/07/17 00:00 [received]
PHST- 2016/10/21 00:00 [accepted]
PHST- 2017/03/30 06:00 [entrez]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/03/30 06:01 [medline]
PHST- 2016/12/16 00:00 [pmc-release]
AID - ETM-0-0-3979 [pii]
AID - 10.3892/etm.2016.3979 [doi]
PST - ppublish
SO  - Exp Ther Med. 2017 Feb;13(2):461-466. doi: 10.3892/etm.2016.3979. Epub 2016 Dec 
      16.