PMID- 28353644
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Mar 29
TI  - Methylmercury Induced Neurotoxicity and the Influence of Selenium in the Brains 
      of Adult Zebrafish (Danio rerio).
LID - 10.3390/ijms18040725 [doi]
LID - 725
AB  - The neurotoxicity of methylmercury (MeHg) is well characterised, and the 
      ameliorating effects of selenium have been described. However, little is known 
      about the molecular mechanisms behind this contaminant-nutrient interaction. We 
      investigated the influence of selenium (as selenomethionine, SeMet) and MeHg on 
      mercury accumulation and protein expression in the brain of adult zebrafish 
      (Danio rerio). Fish were fed diets containing elevated levels of MeHg and/or 
      SeMet in a 2 x 2 full factorial design for eight weeks. Mercury concentrations 
      were highest in the brain tissue of MeHg-exposed fish compared to the controls, 
      whereas lower levels of mercury were found in the brain of zebrafish fed both 
      MeHg and SeMet compared with the fish fed MeHg alone. The expression levels of 
      proteins associated with gap junction signalling, oxidative phosphorylation, and 
      mitochondrial dysfunction were significantly (p < 0.05) altered in the brain of 
      zebrafish after exposure to MeHg and SeMet alone or in combination. Analysis of 
      upstream regulators indicated that these changes were linked to the mammalian 
      target of rapamycin (mTOR) pathways, which were activated by MeHg and inhibited 
      by SeMet, possibly through a reactive oxygen species mediated differential 
      activation of RICTOR, the rapamycin-insensitive binding partner of mTOR.
FAU - Rasinger, Josef Daniel
AU  - Rasinger JD
AD  - National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 
      Nordnes, 5817 Bergen, Norway. josef.rasinger@nifes.no.
FAU - Lundebye, Anne-Katrine
AU  - Lundebye AK
AD  - National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 
      Nordnes, 5817 Bergen, Norway. anne-katrine.lundebye@nifes.no.
FAU - Penglase, Samuel James
AU  - Penglase SJ
AD  - National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 
      Nordnes, 5817 Bergen, Norway. sampenglase@hotmail.com.
AD  - Present address: Aquaculture Research Solutions (ARS), Mundingburra, 4812 QLD, 
      Australia.. sampenglase@hotmail.com.
FAU - Ellingsen, Stale
AU  - Ellingsen S
AD  - National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 
      Nordnes, 5817 Bergen, Norway. stale.ellingsen@uib.no.
AD  - Present address: Department of Biology, University of Bergen, P.O. Box 7803, 5020 
      Bergen, Norway.. stale.ellingsen@uib.no.
FAU - Amlund, Heidi
AU  - Amlund H
AD  - National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029 
      Nordnes, 5817 Bergen, Norway. heidi.amlund@nifes.no.
LA  - eng
PT  - Journal Article
DEP - 20170329
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Methylmercury Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Water Pollutants)
RN  - 964MRK2PEL (Selenomethionine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/metabolism
MH  - Gap Junctions/metabolism
MH  - Methylmercury Compounds/pharmacokinetics/*toxicity
MH  - Mitochondria/drug effects/metabolism
MH  - Oxidative Phosphorylation
MH  - Reactive Oxygen Species/metabolism
MH  - Selenomethionine/pharmacokinetics/*pharmacology
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Water Pollutants/pharmacokinetics/*toxicity
MH  - Zebrafish
PMC - PMC5412311
OTO - NOTNLM
OT  - contaminant
OT  - dietary
OT  - exposure
OT  - interaction
OT  - mechanisms
OT  - mercury
OT  - methylmercury
OT  - nutrient
OT  - proteomics
OT  - selenium
COIS- The authors declare no conflict of interest.
EDAT- 2017/03/30 06:00
MHDA- 2017/04/30 06:00
PMCR- 2017/04/01
CRDT- 2017/03/30 06:00
PHST- 2017/01/31 00:00 [received]
PHST- 2017/03/20 00:00 [revised]
PHST- 2017/03/23 00:00 [accepted]
PHST- 2017/03/30 06:00 [entrez]
PHST- 2017/03/30 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijms18040725 [pii]
AID - ijms-18-00725 [pii]
AID - 10.3390/ijms18040725 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Mar 29;18(4):725. doi: 10.3390/ijms18040725.