PMID- 28357365
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2311-2638 (Print)
IS  - 2311-2638 (Electronic)
IS  - 2311-2638 (Linking)
VI  - 3
IP  - 7
DP  - 2016 Jun 30
TI  - Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis.
PG  - 275-284
LID - 10.15698/mic2016.07.511 [doi]
AB  - Cox23 is a known conserved assembly factor for cytochrome c oxidase, although its 
      role in cytochrome c oxidase (CcO) biogenesis remains unresolved. To gain 
      additional insights into its role, we isolated spontaneous suppressors of the 
      respiratory growth defect in cox23∆ yeast cells. We recovered independent 
      colonies that propagated on glycerol/lactate medium for cox23∆ cells at 37 degrees C. We 
      mapped these mutations to the mitochondrial genome and specifically to COX1 
      yielding an I(101)F substitution. The I(101)F Cox1 allele is a gain-of-function 
      mutation enabling yeast to respire in the absence of Cox23. CcO subunit 
      steady-state levels were restored with the I(101)F Cox1 suppressor mutation and 
      oxygen consumption and CcO activity were likewise restored. Cells harboring the 
      mitochondrial genome encoding I(101)F Cox1 were used to delete genes for other 
      CcO assembly factors to test the specificity of the Cox1 mutation as a suppressor 
      of cox23∆ cells. The Cox1 mutant allele fails to support respiratory growth in 
      yeast lacking Cox17, Cox19, Coa1, Coa2, Cox14 or Shy1, demonstrating its specific 
      suppressor activity for cox23∆ cells.
FAU - Dela Cruz, Richard
AU  - Dela Cruz R
AD  - University of Utah Health Sciences Center, Departments of Medicine and 
      Biochemistry, Salt Lake City, Utah 84132, USA. ; Present address: Oklahoma 
      Medical Research Foundation, Oklahoma City, OK 73104, USA.
FAU - Jeong, Mi-Young
AU  - Jeong MY
AD  - University of Utah Health Sciences Center, Departments of Medicine and 
      Biochemistry, Salt Lake City, Utah 84132, USA.
FAU - Winge, Dennis R
AU  - Winge DR
AD  - University of Utah Health Sciences Center, Departments of Medicine and 
      Biochemistry, Salt Lake City, Utah 84132, USA.
LA  - eng
PT  - Journal Article
DEP - 20160630
PL  - Austria
TA  - Microb Cell
JT  - Microbial cell (Graz, Austria)
JID - 101632887
PMC - PMC5354592
OTO - NOTNLM
OT  - COX1
OT  - COX23
OT  - cytochrome oxidase
OT  - mitochondria
COIS- Conflict of interest: None of the authors have a conflict of interest with this 
      reported study.
EDAT- 2017/03/31 06:00
MHDA- 2017/03/31 06:01
PMCR- 2016/06/30
CRDT- 2017/03/31 06:00
PHST- 2017/03/31 06:00 [entrez]
PHST- 2017/03/31 06:00 [pubmed]
PHST- 2017/03/31 06:01 [medline]
PHST- 2016/06/30 00:00 [pmc-release]
AID - MIC0176E157 [pii]
AID - 10.15698/mic2016.07.511 [doi]
PST - epublish
SO  - Microb Cell. 2016 Jun 30;3(7):275-284. doi: 10.15698/mic2016.07.511.